Gjb2 (NM_008125) Mouse Tagged ORF Clone

CAT#: MG202661

Gjb2 (GFP-tagged) - Mouse gap junction membrane channel protein beta 2 (Gjb2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Gjb2
• Synonyms: AI325222; Cnx26; Cx26; Gjb-2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG202661 representing NM_008125
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTGGGGCACACTCCAGAGCATCCTCGGGGGTGTCAACAAACACTCCACCAGCATTGGAAAGATCT
  GGCTCACGGTCCTCTTCATCTTCCGCATCATGATCCTCGTGGTGGCTGCAAAGGAGGTGTGGGGAGATGA
  GCAAGCCGATTTTGTCTGCAACACGCTCCAGCCTGGCTGCAAGAATGTATGCTACGACCACCACTTCCCC
  ATCTCTCACATCCGGCTCTGGGCTCTGCAGCTGATCATGGTGTCCACGCCAGCCCTCCTGGTAGCTATGC
  ATGTGGCCTACCGGAGACATGAAAAGAAACGGAAGTTCATGAAGGGAGAGATAAAGAACGAGTTTAAGGA
  CATCGAAGAGATCAAAACCCAGAAGGTCCGTATCGAAGGGTCCCTGTGGTGGACCTACACCACCAGCATC
  TTCTTCCGGGTCATCTTTGAAGCCGTCTTCATGTACGTCTTTTACATCATGTACAATGGCTTCTTCATGC
  AACGTCTGGTGAAATGCAACGCTTGGCCCTGCCCCAATACAGTGGACTGCTTCATTTCCAGGCCCACAGA
  AAAGACTGTCTTCACCGTGTTTATGATTTCTGTGTCTGGAATTTGCATTCTGCTAAATATCACAGAGCTG
  TGCTATTTGTTCGTTAGGTATTGCTCAGGAAAGTCCAAAAGACCAGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG202661 representing NM_008125
  Red=Cloning site Green=Tags(s)
  
  MDWGTLQSILGGVNKHSTSIGKIWLTVLFIFRIMILVVAAKEVWGDEQADFVCNTLQPGCKNVCYDHHFP
  ISHIRLWALQLIMVSTPALLVAMHVAYRRHEKKRKFMKGEIKNEFKDIEEIKTQKVRIEGSLWWTYTTSI
  FFRVIFEAVFMYVFYIMYNGFFMQRLVKCNAWPCPNTVDCFISRPTEKTVFTVFMISVSGICILLNITEL
  CYLFVRYCSGKSKRPV
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008125
• ORF Size: 678 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008125.2, NP_032151.1
• RefSeq Size: 2404
• RefSeq ORF: 681
• Locus ID: 14619
• Gene Summary: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. [UniProtKB/Swiss-Prot Function]