Cltb (BC070404) Mouse Tagged ORF Clone

CAT#: MG202747

Cltb (GFP-tagged) - Mouse clathrin, light polypeptide (Lcb) (cDNA clone MGC:92950 IMAGE:5699653)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cltb
• Synonyms: 2310046E19Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG202747 representing BC070404
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGAGGACTTCGGCTTCTTCTCATCGTCGGAGAGCGGGGCCCCGGAGGCCGCCGAGGAGGACCCAG
  CGGCCGCCTTCCTGGCCCAGCAGGAAAGCGAGATTGCTGGCATCGAGAATGACCCGGGTTTCGGGGCACC
  TGCCGCCAGCCAGGTGGCCTCTGCGCAGCCGGGACTCGCGAGCGGGGCTGGTTCAGAGGACATGAGTACT
  ACAGTCAATGGAGATGTGTTTCAGGAGGCTAACGGGCCTGCCGATGGCTACGCTGCGATTGCCCAGGCGG
  ACAGGTTGACTCAGGAGCCCGAGAGCATCCGGAAGTGGAGAGAGGAGCAGAAGAAAAGGCTGCAGGAGTT
  AGACGCTGCCTCGAAGGTGACCGAACAGGAGTGGCGGGAGAAGGCCAAGAAAGACCTGGAGGAGTGGAAC
  CAGCGCCAAAGTGAACAGGTTGAGAAGAACAAGATCAACAACAGGATCGCTGACAAAGCGTTCTACCAGC
  AGCCAGATGCTGATACCATTGGCTATGTGGCATCGGAAGAGGCTTTTGTGAAAGAATCCAAGGAGGAGAC
  CCCGGGCACAGAGTGGGAGAAGGTAGCCCAGCTGTGTGACTTCAATCCCAAGAGCAGCAAGCAGTGTAAA
  GATGTGTCCCGCCTGCGCTCGGTGCTCATGTCCCTGAAGCAAACACCACTGTCCCGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG202747 representing BC070404
  Red=Cloning site Green=Tags(s)
  
  MAEDFGFFSSSESGAPEAAEEDPAAAFLAQQESEIAGIENDPGFGAPAASQVASAQPGLASGAGSEDMST
  TVNGDVFQEANGPADGYAAIAQADRLTQEPESIRKWREEQKKRLQELDAASKVTEQEWREKAKKDLEEWN
  QRQSEQVEKNKINNRIADKAFYQQPDADTIGYVASEEAFVKESKEETPGTEWEKVAQLCDFNPKSSKQCK
  DVSRLRSVLMSLKQTPLSR
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC070404
• ORF Size: 689 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC070404, AAH70404
• RefSeq Size: 1009
• RefSeq ORF: 689
• Locus ID: 74325
• Gene Summary: Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. [UniProtKB/Swiss-Prot Function]