Tpmt (NM_016785) Mouse Tagged ORF Clone

CAT#: MG202963

Tpmt (GFP-tagged) - Mouse thiopurine methyltransferase (Tpmt)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tpmt
• Synonyms: AW106912
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG202963 representing NM_016785
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCTTGATATGAAAGAGCACCCTGATGCTGAGGTACAAAAGAACCAAGTACTAACCCTGGAAGACT
  GGAAAGAGAAGTGGGTGACCCGTCACATCTCATTCCATCAGGAGCAAGGGCATCAGCTATTAAAGAAGCA
  TTTGGATACATTTCTTAAAGGCCAGAGTGGACTGCGAGTGTTTTTCCCCCTCTGTGGAAAAGCCATTGAG
  ATGAAATGGTTCGCAGATCGGGGCCACACTGTAGTTGGTGTGGAAATCAGTGAAATTGGGATTCGGGAAT
  TTTTTGCAGAACAGAATCTTTCCTACACAGAAGAACCGCTCGCCGAAATTGCTGGAGCCAAAGTATTTAA
  GAGTTCTTCAGGGAGCATTTCCTTATACTGCTGCAGCATATTTGATCTTCCTAGAGCAAACATTGGCAAG
  TTTGACAGGATTTGGGATAGAGGAGCATTAGTGGCTATCAATCCAGGCGATCATGATCGCTATGCAGATA
  TAATACTGTCCCTACTGAGAAAAGAGTTTCAGTACCTCGTGGCTGTCCTTTCTTATGATCCAACAAAACA
  CGCAGGCCCACCATTTTATGTTCCATCTGCTGAACTTAAAAGGTTATTTGGTACAAAATGCAGCATGCAA
  TGCCTTGAAGAGGTAGACGCTTTGGAAGAAAGGCATAAAGCCTGGGGTCTTGACTACCTTTTTGAAAAAT
  TGTATCTCCTTACAGAGAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG202963 representing NM_016785
  Red=Cloning site Green=Tags(s)
  
  MSLDMKEHPDAEVQKNQVLTLEDWKEKWVTRHISFHQEQGHQLLKKHLDTFLKGQSGLRVFFPLCGKAIE
  MKWFADRGHTVVGVEISEIGIREFFAEQNLSYTEEPLAEIAGAKVFKSSSGSISLYCCSIFDLPRANIGK
  FDRIWDRGALVAINPGDHDRYADIILSLLRKEFQYLVAVLSYDPTKHAGPPFYVPSAELKRLFGTKCSMQ
  CLEEVDALEERHKAWGLDYLFEKLYLLTEK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016785
• ORF Size: 720 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_016785.1, NP_058065.1
• RefSeq Size: 1068
• RefSeq ORF: 723
• Locus ID: 22017
• Gene Summary: Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine. [UniProtKB/Swiss-Prot Function]