Tpk1 (NM_013861) Mouse Tagged ORF Clone

CAT#: MG203016

Tpk1 (GFP-tagged) - Mouse thiamine pyrophosphokinase (Tpk1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tpk1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG203016 representing NM_013861
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCATGCCTTTACCCCGTTGGAACCCCTGCTACCTACGGGGAACTTGAAATACTGCCTTGTGGTTC
  TTAATCAGCCTTTGGATGCACGATTTCGCCATCTTTGGAAAAAAGCTCTCTTAAGAGCCTGTGCTGATGG
  GGGTGCCAACCACTTATATGATCTCACTGAAGGAGAGAGAGAAAGCTTCTTGCCTGAATTCGTCAGTGGG
  GACTTTGATTCTATTAGGCCTGAAGTCAAAGAGTACTACACCAAGAAGGGCTGTGATCTTATTTCAACTC
  CTGACCAAGACCACACTGACTTTACCAAGTGTCTTCAAGTGCTCCAAAGGAAGATAGAGGAAAAGGAACT
  GCAGGTTGACGTGATTGTGACACTGGGAGGTCTCGGTGGGCGTTTTGACCAAATCATGGCCTCTGTGAAT
  ACCCTTTTCCAAGCCACTCACATCACTCCTGTGCCGATTATAATAATCCAAAAGGACTCTCTCATCTACC
  TCCTCCAACCCGGGAAGCACAGGCTCCATGTAGACACTGGAATGGAAGGGAGCTGGTGTGGCCTGATTCC
  TGTTGGACAGCCTTGCAACCAGGTGACGACAACAGGCCTGAAATGGAACCTCACAAATGATGTTCTTGGC
  TTTGGAACACTGGTCAGTACTTCTAACACCTACGATGGGTCCGGCCTTGTCACTGTGGAAACTGACCACC
  CACTCCTCTGGACCATGGCCATCAAGAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG203016 representing NM_013861
  Red=Cloning site Green=Tags(s)
  
  MEHAFTPLEPLLPTGNLKYCLVVLNQPLDARFRHLWKKALLRACADGGANHLYDLTEGERESFLPEFVSG
  DFDSIRPEVKEYYTKKGCDLISTPDQDHTDFTKCLQVLQRKIEEKELQVDVIVTLGGLGGRFDQIMASVN
  TLFQATHITPVPIIIIQKDSLIYLLQPGKHRLHVDTGMEGSWCGLIPVGQPCNQVTTTGLKWNLTNDVLG
  FGTLVSTSNTYDGSGLVTVETDHPLLWTMAIKS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013861
• ORF Size: 729 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_013861.1, NP_038889.1
• RefSeq Size: 2563
• RefSeq ORF: 732
• Locus ID: 29807