Psme1 (NM_011189) Mouse Tagged ORF Clone

CAT#: MG203169

Psme1 (GFP-tagged) - Mouse proteasome (prosome, macropain) 28 subunit, alpha (Psme1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Psme1
• Synonyms: AW413925; PA28a
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG203169 representing NM_011189
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACACTGAGGGTCCATCCCGAAGCCCAAGCCAAGGTGGATGTGTTCCGTGAAGACCTGTGTAGCA
  AGACAGAGAACCTGCTTGGGAGCTATTTTCCCAAGAAAATCTCAGAGTTGGATGCGTTCTTAAAGGAGCC
  AGCTCTCAATGAAGCCAACCTGAGCAATCTGAAGGCTCCATTGGACATCCCAGTACCCGATCCAGTCAAA
  GAGAAAGAGAAGGAGGAGCGGAAGAAGCAACAGGAGAAGGAAGAGAAGGAAGAGAAGAAGAAAGGGGACG
  AAGACGACAAAGGTCCCCCCTGTGGCCCAGTGAACTGCAATGAGAAGATTGTGGTCCTCCTGCAACGCCT
  AAAACCTGAGATCAAGGATGTCACTGAGCAACTCAATCTGGTCACTACCTGGTTGCAGCTACAGATACCT
  CGGATAGAGGATGGGAATAATTTTGGCGTGGCTGTCCAGGAAAAGGTGTTTGAGCTGATGACCAACCTTC
  ACACCAAGCTGGAAGGCTTCCACACGCAGATCTCCAAGTACTTCTCCGAGAGGGGTGACGCCGTGGCCAA
  AGCAGCCAAGCAGCCCCACGTGGGTGACTATCGGCAGCTGGTGCACGAGCTGGACGAGGCGGAGTACCAG
  GAGATCCGTCTGATGGTCATGGAGATCCGAAATGCTTATGCTGTGTTATATGACATCATCCTGAAGAACT
  TTGAGAAGCTCAAGAAGCCCCGTGGAGAAACCAAGGGAATGATCTAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG203169 representing NM_011189
  Red=Cloning site Green=Tags(s)
  
  MATLRVHPEAQAKVDVFREDLCSKTENLLGSYFPKKISELDAFLKEPALNEANLSNLKAPLDIPVPDPVK
  EKEKEERKKQQEKEEKEEKKKGDEDDKGPPCGPVNCNEKIVVLLQRLKPEIKDVTEQLNLVTTWLQLQIP
  RIEDGNNFGVAVQEKVFELMTNLHTKLEGFHTQISKYFSERGDAVAKAAKQPHVGDYRQLVHELDEAEYQ
  EIRLMVMEIRNAYAVLYDIILKNFEKLKKPRGETKGMIY
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011189
• ORF Size: 747 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_011189.1, NP_035319.1
• RefSeq Size: 977
• RefSeq ORF: 750
• Locus ID: 19186
• Gene Summary: Implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome. [UniProtKB/Swiss-Prot Function]