Bpgm (NM_007563) Mouse Tagged ORF Clone

CAT#: MG203358

Bpgm (GFP-tagged) - Mouse 2,3-bisphosphoglycerate mutase (Bpgm)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Bpgm
• Synonyms: AI323730; AL022789; C86192
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG203358 representing NM_007563
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCAAGCACAAACTTATTATACTAAGACATGGAGAGGGCCAATGGAATAAGGAGAACCGATTCTGTA
  GTTGGGTGGACCAGAAACTTAACAACGACGGACTGGAGGAGGCTCGGAACTGTGGGAGGCAGCTCAAAGC
  TCTCAACTTCGAGTTTGATCTTGTCTTCACATCCATCCTTAACAGGTCCATTCACACAGCCTGGCTGATC
  CTGGAAGAGCTGGGGCAGGAGTGGGTGCCTGTGGAGAGCTCCTGGCGTCTGAATGAGCGTCACTATGGAG
  CCTTGATTGGCCTCAACAGGGAGAAAATGGCTTTGAATCATGGTGAAGAGCAGGTAAGGCTCTGGAGGAG
  AAGCTACAACGTGACCCCACCTCCTATAGAGGAGTCTCATCCCTACTTCCACGAGATCTACAGTGACCGG
  AGGTACAAAGTGTGCGATGTGCCCTTGGACCAACTGCCACGTTCAGAAAGCTTGAAGGATGTTCTGGAGA
  GACTTCTTCCCTACTGGAAGGAAAGGATTGCTCCGGAAATCTTAAAGGGCAAAAGCATTCTGATATCTGC
  TCACGGGAATAGCAGTAGAGCTCTCCTGAAACATCTGGAAGGTATCTCAGACGAGGATATTATCAACATC
  ACTCTGCCCACTGGAGTTCCGATTCTGCTGGAGTTGGATGAGAACCTGCGTGCCGTTGGGCCTCACCAGT
  TCCTGGGCAACCAGGAGGCCATCCAGGCAGCCATTAAGAAAGTGGATGATCAAGGGAAAGTGAAACAAGG
  CAAACAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG203358 representing NM_007563
  Red=Cloning site Green=Tags(s)
  
  MSKHKLIILRHGEGQWNKENRFCSWVDQKLNNDGLEEARNCGRQLKALNFEFDLVFTSILNRSIHTAWLI
  LEELGQEWVPVESSWRLNERHYGALIGLNREKMALNHGEEQVRLWRRSYNVTPPPIEESHPYFHEIYSDR
  RYKVCDVPLDQLPRSESLKDVLERLLPYWKERIAPEILKGKSILISAHGNSSRALLKHLEGISDEDIINI
  TLPTGVPILLELDENLRAVGPHQFLGNQEAIQAAIKKVDDQGKVKQGKQ
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007563
• ORF Size: 777 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_007563.2, NP_031589.1
• RefSeq Size: 2069
• RefSeq ORF: 780
• Locus ID: 12183
• Gene Summary: Plays a major role in regulating hemoglobin oxygen affinity by controlling the levels of its allosteric effector 2,3-bisphosphoglycerate (2,3-BPG). Also exhibits mutase (EC 5.4.2.1) activity. [UniProtKB/Swiss-Prot Function]