Med8 (BC021870) Mouse Tagged ORF Clone

CAT#: MG203549

Med8 (GFP-tagged) - Mouse mediator of RNA polymerase II transcription, subunit 8 homolog (yeast) (cDNA clone MGC:28391 IMAGE:4022630)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Med8
• Synonyms: MNCb-2386, ARC32
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG203549 representing BC021870
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGAGGGAGGAGAAGCAGCTTGAGGCATCATTAGATGCCCTGCTGAATCAAGTGGCCGATCTGAAGA
  ATTCACTGGGGAGTTTCATTTACAAGTTGGAAAACGAGTATGACCGGCTGACCTGGCCCTCTGTCCTGGA
  CAGCTTTGCATTGCTTTCTGGACAGTTGAACACTCTGAACAAGGTCTTAAAGCATGAGAAGACGCCACTG
  TTCCGAAACCAGGTCATCATCCCTCTGGTGCTGTCCCCAGACCGAGACGAAGACCTCATGCGTCAAACTG
  AAGGACGAGTGCCTGTTTTCAGCCATGAGGTCGTCCCTGACCACTTGAGAACCAAGCCTGACCCTGAAGT
  TGAAGAGCAGGAGAAGCAACTGACAACAGATGCTGCCAGAATTGGTGCTGATGCAGCTCAGAAGCAGATC
  CAGAGCTTGAATAAAATGTGCTCAAACCTTTTAGAGAAAATCAGCAAAGAAGAACGAGAATCAGAGAGCG
  GAGGTCTCCGGCCAAACAAGCAGACTTTCAACCCTGGAGACACCAATGCCTTAGTAGCAGCCGTTGCCTT
  TGGGAAGGGGCTATCTAATTGGCGACCTTCAGGTAGCAGTGGTCCTGGGCAACCCGGTCAGCCAGGAGCT
  GGTACAATCCTTGCAGGGGCCTCAGGATTACCGCAGGTTCAGATGCCAGGTGCTCCAAACCAGCAGCAGC
  CAATGCTCAGTGGAGTCCAGATGGCTCAGGCAGGTCAACCAGGGAAAATGCCAAGTGGAATAAAAACCAA
  CATCAAGTCAGCTTCAATGCATCCGTACCAGCGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG203549 representing BC021870
  Red=Cloning site Green=Tags(s)
  
  MQREEKQLEASLDALLNQVADLKNSLGSFIYKLENEYDRLTWPSVLDSFALLSGQLNTLNKVLKHEKTPL
  FRNQVIIPLVLSPDRDEDLMRQTEGRVPVFSHEVVPDHLRTKPDPEVEEQEKQLTTDAARIGADAAQKQI
  QSLNKMCSNLLEKISKEERESESGGLRPNKQTFNPGDTNALVAAVAFGKGLSNWRPSGSSGPGQPGQPGA
  GTILAGASGLPQVQMPGAPNQQQPMLSGVQMAQAGQPGKMPSGIKTNIKSASMHPYQR
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC021870
• ORF Size: 806 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC021870, AAH21870
• RefSeq Size: 1141
• RefSeq ORF: 806
• Locus ID: 80509