Decr2 (NM_011933) Mouse Tagged ORF Clone

CAT#: MG204004

Decr2 (GFP-tagged) - Mouse 2-4-dienoyl-Coenzyme A reductase 2, peroxisomal (Decr2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Decr2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204004 representing NM_011933
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCAGCCGCCGCCCGACGTTGAGGGGGATGACTGTCTTCCTGAATACCACCATCTCTTCTGCCCGG
  ATCTTCTCCAGGACAAAGTGGCCTTTATCACAGGTGGTGGTTCTGGGATTGGCTTCCGGATCGCAGAGAT
  TTTCATGAGGCATGGCTGCCACACTGTCATCGTCGGCAGGAGTCTGCAGAAAGTGACCACGGCTGCTAAG
  AAGTTGGTTGCTGCCACCGGAAAGCGGTGCCTCCCTCTGTCTATGGATGTCCGAGTTCCCCCAGAGGTCA
  TGACTGCTGTGGATCAAGCGCTGCAAGAATTTGGCAAAATCAACATCCTCATTAACTGTGCAGCTGGAAA
  CTTCCTATGCCCTGCCAGTGCTTTGTCTTTCAATGCCTTTAAGACCGTGGTTGACATTGACACCATCGGT
  ACCTTCAATGTGTCTAGTGTGCTTTACAAGAAGTTCTTCCGGGACCATGGAGGAGTGATTGTGAACATTA
  CTGCCACCCTCAGTATGAGGGGGCAGGTGCTACAGCTACATGCAGGCGCTGCCAAAGCAGCTGTGGATGC
  TATGACGCGACACTTGGCTGTGGAATGGGGTCCCCAGAATATCCGTGTCAACAGCCTGGCTCCTGGTGCC
  ATCAGTGGCACTGAGGGTTTAAGGCGATTAAGAGGCTCCAATGCCAGTTCGAAATTAAAGCATTTTTCAA
  ATCCTATTCCAAGACTTGGGACCAAGACAGAGATCGCTCACAGCGTGCTGTACCTGGCCAGCCCTCTGGC
  TTCCTATGTCTCAGGTATCGTGCTGGTGGTTGATGGTGGAAGCTGGATGACATTCCCCAATGGCATCAAG
  CAACTGCTAGAGTTTGAATCCTTCTCTGCTAAGCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204004 representing NM_011933
  Red=Cloning site Green=Tags(s)
  
  MAQPPPDVEGDDCLPEYHHLFCPDLLQDKVAFITGGGSGIGFRIAEIFMRHGCHTVIVGRSLQKVTTAAK
  KLVAATGKRCLPLSMDVRVPPEVMTAVDQALQEFGKINILINCAAGNFLCPASALSFNAFKTVVDIDTIG
  TFNVSSVLYKKFFRDHGGVIVNITATLSMRGQVLQLHAGAAKAAVDAMTRHLAVEWGPQNIRVNSLAPGA
  ISGTEGLRRLRGSNASSKLKHFSNPIPRLGTKTEIAHSVLYLASPLASYVSGIVLVVDGGSWMTFPNGIK
  QLLEFESFSAKL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011933
• ORF Size: 876 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_011933.2, NP_036063.1
• RefSeq Size: 2051
• RefSeq ORF: 879
• Locus ID: 26378
• Gene Summary: Auxiliary enzyme of beta-oxidation. Participates in the degradation of unsaturated fatty enoyl-CoA esters having double bonds in both even- and odd-numbered positions in peroxisome. Catalyzes the NADP-dependent reduction of 2,4-dienoyl-CoA to yield trans-3-enoyl-CoA. Has activity towards short and medium chain 2,4-dienoyl-CoAs, but also towards 2,4,7,10,13,16,19-docosaheptaenoyl-CoA, suggesting that it does not constitute a rate limiting step in the peroxisomal degradation of docosahexaenoic acid. [UniProtKB/Swiss-Prot Function]