Igfbp3 (BC058261) Mouse Tagged ORF Clone

CAT#: MG204018

Igfbp3 (GFP-tagged) - Mouse insulin-like growth factor binding protein 3 (cDNA clone MGC:65287 IMAGE:6487655)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Igfbp3
• Synonyms: IGFBP-3, IGgfbp3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204018 representing BC058261
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATCCCGCGCGCCCCGCGCTCTGGGCGGCTGCGCTCACTGCCCTCACTCTGCTCCGCGGACCGCCGG
  TGGCGCGAGCTGGCGCGGGCGCGGTGGGCGCGGGCCCCGTGGTGCGCTGCGAACCGTGCGACGCGCGTGC
  GCTGTCCCAGTGCGCGCCTCCGCCCACTGCGCCCGCGTGCACGGAGCTGGTGCGAGAGCCCGGCTGCGGC
  TGCTGCCTGACTTGCGCGCTGCGCGAAGGCGACGCGTGCGGCGTCTACACGGAGCGCTGTGGCACCGGCC
  TCCGCTGCCAGCCGCGGCCCGCGGAGCAGTACCCGCTGAGGGCGCTGCTGAATGGCCGCGGGTTCTGCGC
  CAACGCCAGCGCTGCAGGCAGCCTAAGCACCTACCTCCCCTCCCAACCTGCTCCAGGAAACATCAGTGAG
  TCCGAGGAGGAGCACAATGCTGGGAGTGTGGAAAGCCAGGTTGTCCCCAGCACACACCGAGTGACCGATT
  CCAAGTTCCATCCACTCCATGCCAAGATGGATGTCATCAAAAAAGGCCACGCCAGGGACAGCCAGCGCTA
  CAAAGTTGACTATGAGTCTCAAAGCACAGACACCCAGAACTTCTCCTCCGAGTCTAAGCGGGAGACAGAA
  TACGGTCCCTGCCGCAGAGAAATGGAGGACACACTGAATCATCTGAAGTTCCTCAATGTGCTGAGTCCCA
  GAGGCGTCCACATCCCAAACTGTGACAAGAAGGGGTTCTATAAGAAGAAGCAGTGCCGCCCTTCCAAAGG
  CAGAAAGCGGGGCTTCTGCTGGTGTGTGGACAAGTATGGGCAGCCCTTGCCAGGCTACGACACCAAGGGG
  AAAGACGACGTACATTGCCTCAGCGTGCAGAGCCAG
  
  ACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204018 representing BC058261
  Red=Cloning site Green=Tags(s)
  
  MHPARPALWAAALTALTLLRGPPVARAGAGAVGAGPVVRCEPCDARALSQCAPPPTAPACTELVREPGCG
  CCLTCALREGDACGVYTERCGTGLRCQPRPAEQYPLRALLNGRGFCANASAAGSLSTYLPSQPAPGNISE
  SEEEHNAGSVESQVVPSTHRVTDSKFHPLHAKMDVIKKGHARDSQRYKVDYESQSTDTQNFSSESKRETE
  YGPCRREMEDTLNHLKFLNVLSPRGVHIPNCDKKGFYKKKQCRPSKGRKRGFCWCVDKYGQPLPGYDTKG
  KDDVHCLSVQSQ
  
  TRPLE - GFP Tag - V
• Restriction Sites: SgfI-NotI Cloning Scheme for this gene Plasmid Map
• ACCN: BC058261
• ORF Size: 878 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC058261, AAH58261
• RefSeq Size: 2432
• RefSeq ORF: 878
• Locus ID: 16009
• Gene Summary: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R. [UniProtKB/Swiss-Prot Function]