Cldn23 (NM_027998) Mouse Tagged ORF Clone

CAT#: MG204097

Cldn23 (GFP-tagged) - Mouse claudin 23 (Cldn23)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cldn23
• Synonyms: 2310014B08Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204097 representing NM_027998
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGACGCCGGTGGTGATGACGCTGGGCATGGTGCTCACGCCCTGCGGGTTGCTGCTTAATCTTGTCA
  GTACACTGGCCCCGGGCTGGCGGCTGGTGAAGGGCTTTCTGGACCAGCCAGTGGACGTGGTGCTGTACCA
  GGGGCTGTGGGACATATGTCGCGAGCAGAGCAGTCGCGAACGCGAGTGCGGCCAGCCCGACGAGTGGAAC
  TACTTCCAGACCCAGCCTGTGCAGGTGGCCCGGGGACTCATGATCACGTCACTGGCCACTACCGCCCTAG
  GGCTGCTGCTGGCTTCCCTCGGCGTGCGCTGTTGGCAAGATGAGCCCCACTACGGGCTAGCGGGCCTCTC
  TGGCGTGGTGTTTTTCGTCGCTGGCCTCTTCAGCCTCATCCCGGTCTCCTGGTATAACCACTTCTTGTCA
  GATCCCGACGTCCTGGCCGCCCCGAGCTCGCCGGTCACGGTGCAGGTCAGCTACAGTCTGGTGCTGGGCT
  ACCTAGGGAGCTGCCTGCTGCTTCTGGGCGGCTTCTCTCTGGCGCTCAGCTTTGCGCCCTGGTGTGAAGA
  GCGTTGTCGCCGCTGTCGCAAGGCGCCCCCAGCAGGCCCGCGCCGCAGCAGCATCAGCACCGTCTACGTG
  GACTGGCCGGAACCAGCGCTCACACCTGCCATCAAGTACTACAGCGACGGACAGCATCGGCCTCCTCCCA
  CCGCAGAGCACAGGGACACCAGCAAGCTCAAGGTGGGATTCCCGATGCCACGACCGCCACCCAAGTCCTA
  CACCAACCCGATGGATGTGCTTGAGGGAGAAGAAAAGAAGACAGCCACCTCCCAAGGTGGTTCCTCCTCT
  CGCAGCACCCGGCCCTGCCAAAATTCGTTGCCCTGTGACTCTGACCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204097 representing NM_027998
  Red=Cloning site Green=Tags(s)
  
  MRTPVVMTLGMVLTPCGLLLNLVSTLAPGWRLVKGFLDQPVDVVLYQGLWDICREQSSRERECGQPDEWN
  YFQTQPVQVARGLMITSLATTALGLLLASLGVRCWQDEPHYGLAGLSGVVFFVAGLFSLIPVSWYNHFLS
  DPDVLAAPSSPVTVQVSYSLVLGYLGSCLLLLGGFSLALSFAPWCEERCRRCRKAPPAGPRRSSISTVYV
  DWPEPALTPAIKYYSDGQHRPPPTAEHRDTSKLKVGFPMPRPPPKSYTNPMDVLEGEEKKTATSQGGSSS
  RSTRPCQNSLPCDSDL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_027998
• ORF Size: 888 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_027998.2, NP_082274.1
• RefSeq Size: 1835
• RefSeq ORF: 891
• Locus ID: 71908
• Gene Summary: This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This gene is intronless and the protein encoded by this gene is 77% identical to the human homolog. [provided by RefSeq, Aug 2010]