Npl (NM_028749) Mouse Tagged ORF Clone

CAT#: MG204619

Npl (GFP-tagged) - Mouse N-acetylneuraminate pyruvate lyase (Npl)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Npl
• Synonyms: 0610033B02Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204619 representing NM_028749
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTTCCCTAAGAAGAAACTCCGGGGTCTTGTTGCTGCCACCATCACTCCAATGACAGAGAATGGAG
  AAATCAACTTTCCTGTCATTGGTCAGTATGTGGATTACCTGGTGAAGGAACAGGGGGTGAAGAATATTTT
  TGTGAATGGCACCACAGGAGAAGGCCTGTCCCTGAGCGTCTCTGAACGTCGCCAGGTCGCGGAGGAATGG
  GTTAACCAAGGGAGGAACAAGTTGGACCAGGTGGTGATTCACGTGGGAGCACTAAACGTGAAGGAGTCAC
  AAGAACTGGCCCAACATGCAGCAGAAATTGGAGCTGATGGCATTGCTGTCATTGCGCCTTTCTTTTTCAA
  GTCACAGAACAAAGATGCCCTGATAAGCTTCCTGAGAGAGGTGGCAGCCGCAGCTCCTACTTTGCCATTT
  TATTACTACCACATGCCTTCCATGACCGGGGTAAAGATTCGCGCTGAGGAGCTGCTGGACGGGATTCAGG
  ACAAGATCCCCACCTTCCAAGGACTGAAGTTCACCGACACGGACCTCTTGGATTTCGGCCAATGTGTCGA
  TCAGAATCACCAGCGACAGTTTGCTTTGCTCTTTGGGGTGGACGAACAACTGCTGAGTGCTCTGGTCATG
  GGAGCCACTGGAGCAGTAGGCAGTACCTATAACTACCTGGGAAAAAAGACAAACCAGATGTTGGAAGCTT
  TTGAACAGAAGGACTTGGCTTCAGCGCTCAGTTACCAGTTTCGTATCCAGAGATTTATCAACTATGTGAT
  CAAGCTAGGTTTTGGAGTGTCGCAAACCAAAGCCATCATGACGCTGGTCTCTGGGATTCCAATGGGCCCA
  CCCCGGCTTCCTCTGCAGAAAGCCACCCAGGAGTTTACTGCTAAGGCTGAGGCCAAGCTAAAGAGCCTGG
  ATTTCCTTTCTTCCCCTAGTGTAAAGGAGGGGAAGCCTTTAGCTAGTGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204619 representing NM_028749
  Red=Cloning site Green=Tags(s)
  
  MAFPKKKLRGLVAATITPMTENGEINFPVIGQYVDYLVKEQGVKNIFVNGTTGEGLSLSVSERRQVAEEW
  VNQGRNKLDQVVIHVGALNVKESQELAQHAAEIGADGIAVIAPFFFKSQNKDALISFLREVAAAAPTLPF
  YYYHMPSMTGVKIRAEELLDGIQDKIPTFQGLKFTDTDLLDFGQCVDQNHQRQFALLFGVDEQLLSALVM
  GATGAVGSTYNYLGKKTNQMLEAFEQKDLASALSYQFRIQRFINYVIKLGFGVSQTKAIMTLVSGIPMGP
  PRLPLQKATQEFTAKAEAKLKSLDFLSSPSVKEGKPLASA
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_028749
• ORF Size: 960 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_028749.1, NP_083025.1
• RefSeq Size: 1403
• RefSeq ORF: 963
• Locus ID: 74091