Grhpr (NM_080289) Mouse Tagged ORF Clone

CAT#: MG204811

Grhpr (GFP-tagged) - Mouse glyoxylate reductase/hydroxypyruvate reductase (Grhpr)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Grhpr
• Synonyms: 1110059D05Rik; 6430629L09Rik; Glxr
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204811 representing NM_080289
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAACCGGCGCGACTCATGAAGGTGTTCGTCACTGGCCCGTTGCCTGCCGAGGGCAGGGCTGCGCTCG
  CCCAGGCCGCAGACTGTGAGGTGGAACAGTGGAATTCGGATGACCCCATCCCCAGAAAGGATCTGGAGCA
  AGGTGTGGTGGGGGCCCATGGCTTGCTCTGCCGCCTCTCTGACCGTGTGGACAAGAAACTTCTGGATGCC
  GCAGGAGCCAACCTCAGAGTCATCAGCACCTTGTCTGTGGGGGTCGACCACTTGGCTTTGGATGAAATCA
  AGAAGCGCGGGATCCGGGTGGGCTACACGCCAGGTGTCCTGACAGATGCCACTGCAGAACTCGCCGTCTC
  CCTCCTCCTCACCACCTGCCGCCGGTTGCCGGAGGCCATAGAGGAAGTGAAGAACGGCGGCTGGAGCTCC
  TGGAGCCCATTATGGATGTGCGGCTACGGACTCTCGCAGAGCACTGTTGGCATTGTGGGGCTGGGGCGCA
  TAGGTCAGGCCATCGCTCGACGACTGAAACCATTCGGTGTCCAGAGATTTCTTTACACGGGGCGCCAGCC
  CAGGCCTCAGGAAGCAGCCGAGTTTCAGGCAGAGTTTGTGCCTATTGCTCAGCTGGCCGCAGAGTCAGAC
  TTCATTGTCGTGTCCTGCTCCTTAACACCCGATACCATGGGGCTCTGCAGCAAGGATTTCTTCCAGAAGA
  TGAAGAACACAGCTATCTTCATCAACATCAGCAGAGGAGATGTGGTAAACCAGGAAGACCTGTACCAGGC
  ACTAGCCAGTGGTCAGATTGCAGCAGCGGGACTGGATGTGACCACCCCTGAACCACTGCCTCCGAGCCAC
  CCCCTGCTGACCCTCAAGAACTGCGTGATCCTGCCCCACATTGGCAGTGCCACCTACAAAACTCGCAACA
  CCATGTCCTTGCTGGCGGCTAACAACTTGCTGGCTGGCCTAAGAGGGGAGGCCATGCCCAGCGAACTCAA
  GCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204811 representing NM_080289
  Red=Cloning site Green=Tags(s)
  
  MKPARLMKVFVTGPLPAEGRAALAQAADCEVEQWNSDDPIPRKDLEQGVVGAHGLLCRLSDRVDKKLLDA
  AGANLRVISTLSVGVDHLALDEIKKRGIRVGYTPGVLTDATAELAVSLLLTTCRRLPEAIEEVKNGGWSS
  WSPLWMCGYGLSQSTVGIVGLGRIGQAIARRLKPFGVQRFLYTGRQPRPQEAAEFQAEFVPIAQLAAESD
  FIVVSCSLTPDTMGLCSKDFFQKMKNTAIFINISRGDVVNQEDLYQALASGQIAAAGLDVTTPEPLPPSH
  PLLTLKNCVILPHIGSATYKTRNTMSLLAANNLLAGLRGEAMPSELKL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_080289
• ORF Size: 984 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_080289.1, NP_525028.1
• RefSeq Size: 1253
• RefSeq ORF: 987
• Locus ID: 76238
• Gene Summary: This gene encodes a member of the D-isomer specific 2-hydroxyacid dehydrogenase family of proteins. The encoded protein catalyzes three enzymatic reactions: the conversion of hydroxypyruvate to D-glycerate as well as the reverse reaction, and the conversion of glyoxylate to glycolate. Homozygous knockout mice exhibit elevated synthesis of oxalate and glycerate. [provided by RefSeq, Aug 2015]