Mpg (NM_010822) Mouse Tagged ORF Clone

CAT#: MG204931

Mpg (GFP-tagged) - Mouse N-methylpurine-DNA glycosylase (Mpg)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Mpg
• Synonyms: 9830006D05; Aag; AI326268; APNG; Mid1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204931 representing NM_010822
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCGCGCGCGGTGGTAGTGCGCGCCCGGGCAGAGGAGCCCTAAAACCGGTGTCCGTGACCCTGCTCC
  CCGACACCGAGCAGCCTCCATTTCTTGGACGAGCCCGCCGCCCAGGGAATGCCAGAGCAGGATCCCTAGT
  GACCGGATATCATGAGGTCGGCCAGATGCCAGCGCCGCTTTCCCGAAAGATTGGGCAAAAAAAGCAGCGA
  CTGGCAGATTCAGAGCAGCAGCAGACCCCTAAAGAGAGACTCCTGTCGACCCCGGGCCTCCGGCGGAGTA
  TCTACTTCTCCAGCCCAGAGGACCATTCTGGCCGGCTAGGACCAGAGTTTTTTGACCAGCCAGCAGTCAC
  CCTAGCCCGTGCATTTCTGGGACAGGTTCTTGTCCGGCGACTCGCTGATGGAACAGAACTCCGTGGGCGC
  ATTGTGGAGACTGAGGCATACTTGGGGCCAGAAGATGAAGCTGCTCACTCAAGAGGTGGCCGGCAGACCC
  CCCGGAACCGTGGCATGTTCATGAAACCTGGGACCCTGTATGTGTACCTCATCTATGGCATGTACTTCTG
  CTTGAATGTCTCTAGTCAAGGGGCTGGGGCTTGTGTCTTGCTAAGAGCACTAGAGCCCCTGGAGGGCCTG
  GAGACCATGCGGCAGCTTCGTAACTCCCTCCGAAAAAGCACTGTCGGCCGTTCCCTCAAGGACCGTGAAC
  TCTGTAGTGGTCCCTCCAAGCTGTGCCAGGCCCTGGCCATTGATAAGAGCTTTGACCAGCGAGACCTGGC
  TCAAGATGATGCTGTGTGGCTGGAGCATGGCCCTCTGGAGTCCAGTAGCCCAGCTGTGGTGGTGGCAGCA
  GCCCGTATAGGTATTGGTCATGCAGGGGAGTGGACACAGAAGCCCTTGCGCTTCTATGTCCAGGGCAGCC
  CATGGGTCAGTGTGGTAGACAGAGTGGCTGAACAGATGGATCAGCCTCAGCAAACAGCCTGCTCTGAGGG
  GCTTTTAATTGTTCAAAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204931 representing NM_010822
  Red=Cloning site Green=Tags(s)
  
  MPARGGSARPGRGALKPVSVTLLPDTEQPPFLGRARRPGNARAGSLVTGYHEVGQMPAPLSRKIGQKKQR
  LADSEQQQTPKERLLSTPGLRRSIYFSSPEDHSGRLGPEFFDQPAVTLARAFLGQVLVRRLADGTELRGR
  IVETEAYLGPEDEAAHSRGGRQTPRNRGMFMKPGTLYVYLIYGMYFCLNVSSQGAGACVLLRALEPLEGL
  ETMRQLRNSLRKSTVGRSLKDRELCSGPSKLCQALAIDKSFDQRDLAQDDAVWLEHGPLESSSPAVVVAA
  ARIGIGHAGEWTQKPLRFYVQGSPWVSVVDRVAEQMDQPQQTACSEGLLIVQK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010822
• ORF Size: 999 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_010822.3, NP_034952.2
• RefSeq Size: 1745
• RefSeq ORF: 1002
• Locus ID: 268395
• Gene Summary: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions. [UniProtKB/Swiss-Prot Function]