Irf2 (NM_008391) Mouse Tagged ORF Clone

CAT#: MG205275

Irf2 (GFP-tagged) - Mouse interferon regulatory factor 2 (Irf2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Irf2
• Synonyms: 9830146E22Rik; AI646973; Irf-2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205275 representing NM_008391
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGTGGAACGGATGCGAATGCGCCCGTGGCTGGAGGAGCAGATAAATTCCAATACGATACCAGGGC
  TAAAGTGGCTGAACAAGGAGAAGAAGATTTTCCAGATCCCCTGGATGCATGCGGCTCGGCACGGATGGGA
  CGTGGAAAAGGATGCTCCGCTCTTCAGAAACTGGGCGATCCATACAGGAAAGCATCAACCAGGAATAGAT
  AAACCAGATCCAAAAACATGGAAAGCAAATTTTCGATGTGCCATGAATTCCCTGCCCGACATTGAGGAAG
  TGAAGGACAGAAGCATAAAGAAAGGAAACAACGCCTTCAGAGTCTACCGGATGCTGCCCTTATCCGAACG
  ACCTTCCAAGAAAGGAAAGAAACCAAAGACAGAAAAAGAAGAGAGAGTTAAGCACATCAAGCAAGAACCA
  GTTGAGTCATCTTTGGGGCTTAGTAATGGAGTAAGTGGCTTTTCTCCTGAGTATGCGGTCCTGACTTCAG
  CTATAAAAAATGAAGTGGATAGTACGGTGAACATCATAGTTGTAGGACAGTCCCATCTGGACAGCAACAT
  TGAAGATCAAGAGATCGTCACTAACCCGCCAGACATCTGCCAGGTTGTAGAAGTGACCACTGAGAGTGAT
  GACCAGCCAGTCAGCATGAGTGAGCTCTACCCTCTACAGATTTCTCCTGTGTCTTCCTACGCAGAAAGCG
  AAACTACCGACAGTGTGGCCAGTGATGAAGAGAACGCAGAGGGGAGACCACACTGGAGGAAGAGGAGCAT
  CGAAGGCAAGCAGTACCTCAGCAACATGGGGACACGGAACACCTATCTGCTGCCCAGCATGGCGACCTTT
  GTCACCTCCAACAAGCCAGATCTGCAGGTCACCATCAAAGAGGATAGCTGTCCGATGCCTTACAACAGCT
  CCTGGCCCCCATTTACAGACCTTCCCCTTCCTGCCCCAGTGACCCCCACGCCCAGCAGCAGTCGGCCAGA
  CCGGGAGACCCGGGCCAGTGTCATCAAGAAGACATCTGATATCACCCAGGCCCGTGTCAAGAGCTGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205275 representing NM_008391
  Red=Cloning site Green=Tags(s)
  
  MPVERMRMRPWLEEQINSNTIPGLKWLNKEKKIFQIPWMHAARHGWDVEKDAPLFRNWAIHTGKHQPGID
  KPDPKTWKANFRCAMNSLPDIEEVKDRSIKKGNNAFRVYRMLPLSERPSKKGKKPKTEKEERVKHIKQEP
  VESSLGLSNGVSGFSPEYAVLTSAIKNEVDSTVNIIVVGQSHLDSNIEDQEIVTNPPDICQVVEVTTESD
  DQPVSMSELYPLQISPVSSYAESETTDSVASDEENAEGRPHWRKRSIEGKQYLSNMGTRNTYLLPSMATF
  VTSNKPDLQVTIKEDSCPMPYNSSWPPFTDLPLPAPVTPTPSSSRPDRETRASVIKKTSDITQARVKSC
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008391
• ORF Size: 1047 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008391.4, NP_032417.3
• RefSeq Size: 2484
• RefSeq ORF: 1050
• Locus ID: 16363
• Gene Summary: Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) and represses those genes. Also acts as an activator for several genes including H4 and IL7. Constitutively binds to the ISRE promoter to activate IL7. Involved in cell cycle regulation through binding the site II (HiNF-M) promoter region of H4 and activating transcription during cell growth. Antagonizes IRF1 transcriptional activation. [UniProtKB/Swiss-Prot Function]