Wnt7a (NM_009527) Mouse Tagged ORF Clone

CAT#: MG205285

Wnt7a (GFP-tagged) - Mouse wingless-related MMTV integration site 7A (Wnt7a)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Wnt7a
• Synonyms: AI849442; px; tw; Wnt-7a
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205285 representing NM_009527
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCCGGAAAGCGCGGCGCTGCCTGGGCCACCTCTTTCTCAGCCTGGGCATAGTCTACCTCCGGATCG
  GTGGCTTCTCTTCGGTGGTAGCTCTGGGTGCGAGCATCATCTGTAACAAGATCCCAGGCCTGGCTCCCAG
  ACAGCGGGCAATCTGCCAGAGCCGGCCGGACGCCATCATCGTCATAGGAGAAGGCTCCCAAATGGGCCTG
  GACGAGTGTCAGTTTCAGTTCCGAAATGGCCGTTGGAACTGCTCAGCGCTGGGAGAGCGTACTGTCTTCG
  GGAAGGAGCTCAAAGTGGGGAGTCGGGAGGCTGCCTTCACCTATGCGATTATCGCTGCGGGCGTGGCCCA
  TGCCATCACTGCTGCCTGCACCCAGGGCAACCTGAGCGACTGTGGCTGCGACAAGGAGAAGCAAGGCCAG
  TACCACCGGGACGAGGGCTGGAAGTGGGGTGGCTGCTCTGCCGACATCCGCTACGGCATCGGCTTCGCCA
  AGGTCTTCGTGGATGCCCGGGAGATCAAGCAGAATGCCCGGACGCTCATGAACTTACACAATAACGAGGC
  GGGTCGGAAGATCCTGGAGGAGAACATGAAGCTGGAGTGTAAGTGCCATGGTGTGTCAGGCTCCTGTACC
  ACTAAGACGTGCTGGACCACACTGCCACAGTTCCGAGAGCTAGGCTACGTGCTCAAGGACAAATACAACG
  AGGCCGTCCACGTGGAGCCTGTGCGTGCCAGTCGAAACAAGCGGCCCACCTTTCTGAAGATCAAGAAGCC
  CCTGTCCTACCGCAAGCCCATGGACACTGACCTGGTGTATATCGAGAAGTCACCCAATTACTGTGAAGAG
  GACCCAGTGACAGGCAGCGTGGGTACCCAGGGCCGAGCCTGCAATAAGACAGCCCCTCAGGCCAGTGGCT
  GTGACCTCATGTGCTGTGGCCGTGGCTACAACACACACCAGTACGCCCGGGTGTGGCAGTGCAACTGCAA
  ATTCCACTGGTGCTGCTACGTCAAGTGTAACACGTGCAGCGAGCGCACGGAGATGTATACGTGCAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205285 representing NM_009527
  Red=Cloning site Green=Tags(s)
  
  MTRKARRCLGHLFLSLGIVYLRIGGFSSVVALGASIICNKIPGLAPRQRAICQSRPDAIIVIGEGSQMGL
  DECQFQFRNGRWNCSALGERTVFGKELKVGSREAAFTYAIIAAGVAHAITAACTQGNLSDCGCDKEKQGQ
  YHRDEGWKWGGCSADIRYGIGFAKVFVDAREIKQNARTLMNLHNNEAGRKILEENMKLECKCHGVSGSCT
  TKTCWTTLPQFRELGYVLKDKYNEAVHVEPVRASRNKRPTFLKIKKPLSYRKPMDTDLVYIEKSPNYCEE
  DPVTGSVGTQGRACNKTAPQASGCDLMCCGRGYNTHQYARVWQCNCKFHWCCYVKCNTCSERTEMYTCK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009527
• ORF Size: 1047 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009527.2, NP_033553.2
• RefSeq Size: 3176
• RefSeq ORF: 1050
• Locus ID: 22421