Prkaca (NM_008854) Mouse Tagged ORF Clone

CAT#: MG205322

Prkaca (GFP-tagged) - Mouse protein kinase, cAMP dependent, catalytic, alpha (Prkaca)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Prkaca
• Synonyms: Pkaca; PKCD
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205322 representing NM_008854
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAACGCCGCCGCCGCCAAGAAGGGCAGCGAGCAGGAGAGCGTGAAAGAGTTCCTAGCCAAAGCCA
  AGGAAGATTTCCTGAAAAAATGGGAGACCCCTTCTCAGAATACAGCCCAGTTGGATCAGTTTGATAGAAT
  CAAGACCCTTGGCACCGGCTCCTTTGGGCGAGTGATGCTGGTGAAGCACAAGGAGAGTGGGAACCACTAC
  GCCATGAAGATCTTAGACAAGCAGAAGGTGGTGAAGCTAAAGCAGATCGAGCACACTCTGAATGAGAAGC
  GCATCCTGCAGGCCGTCAACTTCCCGTTCCTGGTCAAACTTGAATTCTCCTTCAAGGACAACTCAAACCT
  GTACATGGTCATGGAGTATGTAGCTGGTGGCGAGATGTTCTCCCACCTACGGCGGATTGGAAGGTTCAGC
  GAGCCCCATGCCCGTTTCTACGCGGCGCAGATCGTCCTGACCTTTGAGTATCTGCACTCCCTGGACCTCA
  TCTACCGGGACCTGAAGCCCGAGAATCTTCTCATCGACCAGCAGGGCTATATTCAGGTGACAGACTTCGG
  TTTTGCCAAGCGTGTGAAAGGCCGTACTTGGACCTTGTGTGGGACCCCTGAGTACTTGGCCCCCGAGATT
  ATCCTGAGCAAAGGCTACAACAAGGCTGTGGACTGGTGGGCTCTCGGAGTCCTCATCTACGAGATGGCTG
  CTGGTTACCCACCCTTCTTCGCTGACCAGCCTATCCAGATCTATGAGAAAATCGTCTCTGGGAAGGTGCG
  GTTCCCATCCCACTTCAGCTCTGACTTGAAGGACCTGCTGCGGAACCTTCTGCAGGTGGATCTCACCAAG
  CGCTTTGGGAACCTCAAGAACGGGGTCAATGACATCAAGAACCACAAGTGGTTTGCCACGACTGACTGGA
  TTGCCATCTATCAGAGAAAGGTGGAAGCTCCCTTCATACCAAAGTTTAAAGGCCCTGGGGACACGAGTAA
  CTTTGACGACTATGAGGAGGAAGAGATCCGGGTCTCCATCAATGAGAAGTGTGGCAAGGAGTTTACTGAG
  TTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205322 representing NM_008854
  Red=Cloning site Green=Tags(s)
  
  MGNAAAAKKGSEQESVKEFLAKAKEDFLKKWETPSQNTAQLDQFDRIKTLGTGSFGRVMLVKHKESGNHY
  AMKILDKQKVVKLKQIEHTLNEKRILQAVNFPFLVKLEFSFKDNSNLYMVMEYVAGGEMFSHLRRIGRFS
  EPHARFYAAQIVLTFEYLHSLDLIYRDLKPENLLIDQQGYIQVTDFGFAKRVKGRTWTLCGTPEYLAPEI
  ILSKGYNKAVDWWALGVLIYEMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTK
  RFGNLKNGVNDIKNHKWFATTDWIAIYQRKVEAPFIPKFKGPGDTSNFDDYEEEEIRVSINEKCGKEFTE
  F
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008854
• ORF Size: 1053 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008854.3, NP_032880.1
• RefSeq Size: 2292
• RefSeq ORF: 1056
• Locus ID: 18747
• Gene Summary: This gene encodes a member of the serine/threonine protein kinase family. The holoenzyme, protein kinase A (also known as cyclic-AMP dependent protein kinase), mediates cellular response to changes in cyclic-AMP levels. This gene encodes the alpha catalytic subunit of protein kinase A. Protein kinase A-mediated signaling is transduced via phosphorylation of target proteins, and is important for many cellular functions, including mammalian sperm maturation and motility. Alternative splicing results in multiple transcript variants. A pseudogene of this gene has been defined on the X chromosome. [provided by RefSeq, Apr 2013]