Cysltr1 (NM_021476) Mouse Tagged ORF Clone

CAT#: MG205348

Cysltr1 (GFP-tagged) - Mouse cysteinyl leukotriene receptor 1 (Cysltr1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cysltr1
• Synonyms: BB147369; Cyslt1; CysLT1R
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205348 representing NM_021476
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACCTCCAAGGCACCAAGCAGACATTCCTGGAGAACATGAATGGAACTGAAAATCTGACGACATCTC
  TCATTAATAACACGTGTCATGACACAATTGATGAATTCCGAAATCAAGTATACTCCACTATGTATTCTGT
  GATCTCTGTTGTGGGTTTCTTTGGCAATAGCTTTGTGCTCTATGTCCTCATAAAAACATACCATGAGAAA
  TCAGCCTTCCAAGTATACATGATTAATCTAGCCATAGCAGATCTACTCTGTGTATGTACATTGCCTCTCC
  GTGTGGTCTATTATGTTCACAAAGGCAAGTGGCTCTTTGGTGACTTTTTGTGCCGCCTCACCACCTATGC
  CTTGTACGTTAACCTCTATTGTAGCATCTTCTTTATGACAGCCATGAGCTTTTTCCGGTGTGTTGCAATT
  GTCTTTCCAGTCCAGAACATTAATTTGGTTACACAGAAAAAAGCCAGGTTCGTTTGCATTGGAATTTGGA
  TTTTTGTGATTTTGACAAGTTCTCCCTTTTTAATGTACAAATCTTACCAAGATGAGAAAAACAATACTAA
  ATGCTTTGAGCCTCCACAGAACAATCAAGCTAAAAAATACGTTTTGATCTTGCATTATGTGTCATTATTC
  TTTGGTTTCATCATCCCTTTTGTCACCATAATTGTCTGTTACACAATGATCATTCTGACCTTACTAAAAA
  ATACAATGAAGAAAAACATGCCAAGTCGTAGGAAGGCTATAGGGATGATCATAGTTGTGACAGCTGCCTT
  TTTAGTGAGCTTCATGCCATATCATATTCAACGAACTATCCACCTTCACCTTTTACACAGTGAAACTAGA
  CCCTGTGATTCTGTCCTTAGGATGCAGAAGTCAGTGGTCATAACCTTATCTCTAGCTGCATCAAATTGTT
  GCTTTGATCCTCTGCTATATTTCTTTTCAGGTGGAAACTTTAGGAGAAGGCTATCTACATTTAGAAAGCA
  TTCTTTGTCCAGTATGACTTATGTACCCAAGAAGAAAGCTTCCTTGCCAGAAAAAGGAGAAGAAATATGT
  AACGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205348 representing NM_021476
  Red=Cloning site Green=Tags(s)
  
  MYLQGTKQTFLENMNGTENLTTSLINNTCHDTIDEFRNQVYSTMYSVISVVGFFGNSFVLYVLIKTYHEK
  SAFQVYMINLAIADLLCVCTLPLRVVYYVHKGKWLFGDFLCRLTTYALYVNLYCSIFFMTAMSFFRCVAI
  VFPVQNINLVTQKKARFVCIGIWIFVILTSSPFLMYKSYQDEKNNTKCFEPPQNNQAKKYVLILHYVSLF
  FGFIIPFVTIIVCYTMIILTLLKNTMKKNMPSRRKAIGMIIVVTAAFLVSFMPYHIQRTIHLHLLHSETR
  PCDSVLRMQKSVVITLSLAASNCCFDPLLYFFSGGNFRRRLSTFRKHSLSSMTYVPKKKASLPEKGEEIC
  NE
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021476
• ORF Size: 2976 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_021476.4
• RefSeq Size: 2754
• RefSeq ORF: 1059
• Locus ID: 58861
• Gene Summary: Receptor for cysteinyl leukotrienes mediating constriction of the microvascular smooth muscle during an inflammatory response. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The rank order of affinities for the leukotrienes is LTD4 >> LTE4 = LTC4 >> LTB4. [UniProtKB/Swiss-Prot Function]