Cebpa (NM_007678) Mouse Tagged ORF Clone

CAT#: MG205482

Cebpa (GFP-tagged) - Mouse CCAAT/enhancer binding protein (C/EBP), alpha (Cebpa)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cebpa
• Synonyms: C/ebpalpha; CBF-A; Cebp
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205482 representing NM_007678
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTCGGCCGACTTCTACGAGGTGGAGCCGCGGCCCCCGATGAGCAGTCACCTCCAGAGCCCCCCGC
  ACGCGCCCAGCAACGCCGCCTTTGGCTTTCCCCGGGGCGCGGGCCCCGCGCCGCCCCCAGCCCCACCTGC
  CGCCCCGGAGCCGCTGGGCGGCATCTGCGAGCACGAGACGTCTATAGACATCAGCGCCTACATCGACCCG
  GCCGCCTTCAACGACGAGTTCCTGGCCGACCTCTTCCAGCACAGCCGACAGCAGGAGAAGGCCAAGGCGG
  CGGCGGGCCCCGCGGGTGGCGGCGGTGACTTTGACTACCCGGGAGCCCCGGCGGGCCCCGGCGGCGCGGT
  CATGTCCGCGGGGGCGCACGGGCCCCCTCCCGGCTACGGCTGTGCGGCGGCCGGCTACCTGGACGGCAGG
  CTGGAGCCCCTGTACGAGCGCGTCGGGGCGCCCGCGCTACGGCCGCTGGTGATCAAACAAGAGCCCCGCG
  AGGAGGACGAGGCGAAGCAGCTGGCGCTGGCCGGCCTCTTCCCCTACCAGCCACCGCCGCCACCGCCACC
  GCCGCACCCGCACGCGTCTCCCGCGCACCTGGCCGCCCCCCACTTGCAGTTCCAGATCGCGCACTGCGGC
  CAGACCACCATGCACCTGCAGCCTGGCCACCCCACACCGCCGCCCACGCCCGTGCCCAGCCCGCACGCTG
  CGCCCGCCTTGGGTGCTGCGGGCCTGCCTGGCCCCGGGAGCGCGCTCAAGGGCTTGGCCGGTGCGCACCC
  CGACCTCCGCACGGGAGGCGGCGGCGGTGGCAGCGGTGCCGGTGCGGGCAAAGCCAAGAAGTCGGTGGAC
  AAGAACAGCAACGAGTACCGGGTACGGCGGGAACGCAACAACATCGCGGTGCGCAAGAGCCGAGATAAAG
  CCAAACAACGCAACGTGGAGACGCAACAGAAGGTGCTGGAGTTGACCAGTGACAATGACCGCCTGCGCAA
  GCGGGTGGAACAGCTGAGCCGTGAACTGGACACGCTGCGGGGCATCTTCCGCCAGCTGCCTGAGAGCTCC
  TTGGTCAAGGCCATGGGCAACTGCGCG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205482 representing NM_007678
  Red=Cloning site Green=Tags(s)
  
  MESADFYEVEPRPPMSSHLQSPPHAPSNAAFGFPRGAGPAPPPAPPAAPEPLGGICEHETSIDISAYIDP
  AAFNDEFLADLFQHSRQQEKAKAAAGPAGGGGDFDYPGAPAGPGGAVMSAGAHGPPPGYGCAAAGYLDGR
  LEPLYERVGAPALRPLVIKQEPREEDEAKQLALAGLFPYQPPPPPPPPHPHASPAHLAAPHLQFQIAHCG
  QTTMHLQPGHPTPPPTPVPSPHAAPALGAAGLPGPGSALKGLAGAHPDLRTGGGGGGSGAGAGKAKKSVD
  KNSNEYRVRRERNNIAVRKSRDKAKQRNVETQQKVLELTSDNDRLRKRVEQLSRELDTLRGIFRQLPESS
  LVKAMGNCA
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007678
• ORF Size: 1077 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_007678.3, NP_031704.2
• RefSeq Size: 2651
• RefSeq ORF: 1080
• Locus ID: 12606
• Gene Summary: This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. The use of alternative in-frame non-AUG (CUG) and AUG start codons results in several protein isoforms with different lengths. Differential translation initiation is mediated by an out-of-frame, upstream open reading frame which is located between the CUG and the first AUG start codons. [provided by RefSeq, Sep 2014]