St8sia4 (NM_009183) Mouse Tagged ORF Clone

CAT#: MG205502

St8sia4 (GFP-tagged) - Mouse ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4 (St8sia4)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: St8sia4
• Synonyms: PST; PST-1; SIAT8-D; Siat8d; ST8SiaIV
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205502 representing NM_009183
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCTCAATTAGAAAACGGTGGACCATCTGCACTATAAGTCTACTCCTGATCTTTTATAAGACAAAAG
  AAATAGCCAGAACTGAGGAGCACCAAGAGACGCAACTCATCGGAGATGGTGAACTGTGTTTGAGCAGATC
  ACTTGTCAACAGCTCTGATAAAATCATCCGGAAGGCTGGCTCCACCATCTTCCAACATTCTGTACAAGGC
  TGGAAAATCAATTCTTCTTTAGTCCTGGAGATAAGGAAGAACATTCTTCGTTTCTTAGATGCGGAACGTG
  ACGTATCTGTGGTCAAGAGCAGTTTTAAGCCTGGTGATGTCATACACTATGTGCTGGACAGACGCCGGAC
  ACTAAATATTTCTCACAATCTGCATAGCCTCCTGCCTGAAGTTTCACCAATGAAAAACCGCAGGTTTAAG
  ACCTGTGCAGTTGTTGGAAATTCCGGCATTCTGCTAGACAGTGGATGTGGAAAGGAGATTGACAGCCACA
  ACTTTGTAATAAGGTGCAATCTAGCTCCTGTGGTGGAGTTCGCTGCTGATGTGGGGACTAAATCAGATTT
  TATTACCATGAATCCATCAGTTGTGCAAAGAGCATTTGGAGGCTTTCGGAATGAGAGTGACAGAGAAAAA
  TTTGTGCATAGACTCTCCATGCTGAATGACAGCGTCCTTTGGATTCCTGCCTTCATGGTCAAAGGAGGAG
  AGAAGCACGTGGAGTGGGTTAATGCATTAATCCTTAAGAACAAACTGCAAGTGCGAACTGCCTATCCATC
  ACTTAGACTTATTCATGCAGTCAGAGGATACTGGCTGACTAACAAAGTTCCTATCAAAAGACCTAGCACA
  GGTCTCCTCATGTATACACTTGCCACCAGATTCTGTGATGAAATTCACCTTTATGGATTCTGGCCATTTC
  CCAAGGATCTGAATGGAAAAGCTGTCAAATATCATTACTATGATGACTTAAAATATAGATACTTTTCCAA
  CGCCAGTCCTCACAGGATGCCATTAGAATTTAAAACATTGAGTGTGCTACACAATAGAGGAGCTCTAAAA
  CTGACCACAGGAAAGTGCATGAAGCAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205502 representing NM_009183
  Red=Cloning site Green=Tags(s)
  
  MRSIRKRWTICTISLLLIFYKTKEIARTEEHQETQLIGDGELCLSRSLVNSSDKIIRKAGSTIFQHSVQG
  WKINSSLVLEIRKNILRFLDAERDVSVVKSSFKPGDVIHYVLDRRRTLNISHNLHSLLPEVSPMKNRRFK
  TCAVVGNSGILLDSGCGKEIDSHNFVIRCNLAPVVEFAADVGTKSDFITMNPSVVQRAFGGFRNESDREK
  FVHRLSMLNDSVLWIPAFMVKGGEKHVEWVNALILKNKLQVRTAYPSLRLIHAVRGYWLTNKVPIKRPST
  GLLMYTLATRFCDEIHLYGFWPFPKDLNGKAVKYHYYDDLKYRYFSNASPHRMPLEFKTLSVLHNRGALK
  LTTGKCMKQ
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009183
• ORF Size: 1077 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009183.1, NP_033209.1
• RefSeq Size: 6786
• RefSeq ORF: 1080
• Locus ID: 20452
• Gene Summary: Catalyzes the polycondensation of alpha-2,8-linked sialic acid required for the synthesis of polysialic acid (PSA), which is present on the embryonic neural cell adhesion molecule (N-CAM), necessary for plasticity of neural cells. [UniProtKB/Swiss-Prot Function]