Aldob (NM_144903) Mouse Tagged ORF Clone

CAT#: MG205585

Aldob (GFP-tagged) - Mouse aldolase 2, B isoform (Aldob)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Aldob
• Synonyms: Aldo-2; Aldo2; BC016435
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205585 representing NM_144903
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCACCGATTTCCAGCCCTCACCCCAGAGCAGAAGAAGGAGCTCTCTGAAATTGCCCAGCGCATTG
  TTGCCAATGGGAAGGGCATCCTGGCTGCAGATGAATCTGTGGGTACCATGGGAAACCGCCTGCAAAGGAT
  AAAGGTGGAGAACACCGAAGAGAACCGAAGGCAGTTCCGAGAACTCCTCTTTAGTGTGGACAATTCCATT
  AGCCAGAGCATCGGCGGAGTGATCCTTTTCCACGAGACCCTCTACCAGAAGGACAGCCAGGGAAATCTGT
  TCAGAAACGTTCTCAAGGAGAAGGGAATTGTGGTGGGCATCAAGTTGGACCAAGGAGGTGCCCCGCTTGC
  AGGAACAAACAAGGAAACCACCATTCAAGGGCTTGATGGCCTCTCTGAACGCTGTGCTCAGTACAAGAAA
  GATGGAGTTGACTTTGGGAAGTGGCGTGCTGTGTTGAGGATTGCTGACCAGTGTCCTTCCAGCCTTGCTA
  TCCAAGAAAATGCCAATGCTCTGGCTCGCTATGCCAGCATCTGTCAGCAGAATGGGCTGGTCCCTATTGT
  TGAGCCAGAGGTGCTTCCTGACGGAGACCATGACCTGGAGCACTGCCAGTATGTTTCTGAGAAGGTCCTG
  GCTGCTGTCTACAAGGCTCTCAATGATCATCATGTTTACCTAGAAGGCACCTTGCTAAAGCCAAACATGG
  TGACTGCTGGACATGCCTGCACGAAGAAGTATACACCAGAGCAGGTGGCTATGGCCACTGTCACAGCTCT
  CCACAGAACTGTTCCTGCAGCTGTTCCTGGTATCTGCTTTTTGTCTGGAGGTATGAGTGAGGAGGATGCT
  ACACTTAACCTCAATGCTATCAACCGTTGCCCTCTACCAAGGCCCTGGAAACTAAGCTTTTCATATGGAA
  GAGCCCTCCAGGCCAGTGCATTGGCTGCCTGGGGTGGCAAGGCTGCAAACAAGAAGGCAACCCAGGAAGC
  TTTCATGAAGCGGGCTATGGCTAACTGCCAGGCGGCCCAAGGACAGTACGTTCACACAGGCTCTTCAGGT
  GCTGCTGCCACCCAGTCGCTCTTCACAGCCTCCTACACCTAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205585 representing NM_144903
  Red=Cloning site Green=Tags(s)
  
  MAHRFPALTPEQKKELSEIAQRIVANGKGILAADESVGTMGNRLQRIKVENTEENRRQFRELLFSVDNSI
  SQSIGGVILFHETLYQKDSQGNLFRNVLKEKGIVVGIKLDQGGAPLAGTNKETTIQGLDGLSERCAQYKK
  DGVDFGKWRAVLRIADQCPSSLAIQENANALARYASICQQNGLVPIVEPEVLPDGDHDLEHCQYVSEKVL
  AAVYKALNDHHVYLEGTLLKPNMVTAGHACTKKYTPEQVAMATVTALHRTVPAAVPGICFLSGGMSEEDA
  TLNLNAINRCPLPRPWKLSFSYGRALQASALAAWGGKAANKKATQEAFMKRAMANCQAAQGQYVHTGSSG
  AAATQSLFTASYTY
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144903
• ORF Size: 1092 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_144903.2, NP_659152.1
• RefSeq Size: 1993
• RefSeq ORF: 1095
• Locus ID: 230163
• Gene Summary: This gene encodes a subunit of the homotetrameric enzyme aldolase B, an isozyme of the class I fructose 1,6-bisphosphate aldolase enzyme. This enzyme catalyzes the conversion of fructose 1,6-bisphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate. Homozygous knockout mice for this gene exhibit liver damage and death following fructose ingestion. A pseudogene of this gene has been identified in the genome. [provided by RefSeq, Aug 2015]