Dusp1 (NM_013642) Mouse Tagged ORF Clone

CAT#: MG205652

Dusp1 (GFP-tagged) - Mouse dual specificity phosphatase 1 (Dusp1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Dusp1
• Synonyms: 3CH134; erp; mkp-1; MKP1; Ptpn16; U19515
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205652 representing NM_013642
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGATGGAGGTGGGCATCCTGGACGCCGGGGGGCTGCGCGCGCTGCTGCGAGAGGGCGCCGCGCAGT
  GCCTGTTGTTGGATTGTCGCTCCTTCTTCGCTTTCAACGCCGGCCACATCGCGGGCTCAGTGAACGTGCG
  CTTCAGCACCATCGTGCGTCGCCGCGCCAAGGGCGCCATGGGCCTGGAGCATATCGTGCCCAACGCTGAA
  CTGCGTGGCCGCCTGCTGGCCGGAGCCTACCACGCCGTGGTGCTGCTGGACGAGCGCAGCGCCTCCCTGG
  ACGGCGCCAAGCGCGACGGCACCCTGGCCCTGGCCGCGGGCGCGCTCTGCCGAGAGGCGCGCTCCACTCA
  AGTCTTCTTTCTCCAAGGAGGATATGAAGCGTTTTCGGCTTCCTGCCCTGAGCTGTGCAGCAAACAGTCC
  ACCCCCACGGGGCTCAGCCTCCCCCTGAGTACTAGTGTGCCTGACAGTGCAGAATCCGGATGCAGCTCCT
  GTAGTACCCCTCTCTACGATCAGGGGGGCCCAGTGGAGATCCTGTCCTTCCTGTACCTGGGCAGTGCCTA
  TCACGCTTCTCGGAAGGATATGCTTGACGCCTTGGGCATCACCGCCTTGATCAACGTCTCAGCCAATTGT
  CCTAACCACTTTGAGGGTCACTACCAGTACAAGAGCATCCCTGTGGAGGACAACCACAAGGCAGACATCA
  GCTCCTGGTTCAACGAGGCTATTGACTTCATAGACTCCATCAAGGATGCTGGAGGGAGAGTGTTTGTTCA
  TTGCCAGGCCGGCATCTCCCGGTCAGCCACCATCTGCCTTGCTTACCTCATGAGGACTAACCGGGTAAAG
  CTGGACGAGGCCTTTGAGTTTGTGAAGCAGAGGCGGAGTATCATCTCCCCGAACTTCAGCTTCATGGGCC
  AGCTGCTGCAGTTTGAGTCCCAAGTGCTAGCCCCTCACTGCTCTGCTGAAGCTGGGAGCCCTGCCATGGC
  TGTCCTTGACCGGGGCACCTCTACTACCACAGTCTTCAACTTCCCTGTCTCCATCCCCGTCCACCCCACG
  AACAGTGCCCTGAACTACCTTAAAAGCCCCATCACCACCTCTCCAAGCTGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205652 representing NM_013642
  Red=Cloning site Green=Tags(s)
  
  MVMEVGILDAGGLRALLREGAAQCLLLDCRSFFAFNAGHIAGSVNVRFSTIVRRRAKGAMGLEHIVPNAE
  LRGRLLAGAYHAVVLLDERSASLDGAKRDGTLALAAGALCREARSTQVFFLQGGYEAFSASCPELCSKQS
  TPTGLSLPLSTSVPDSAESGCSSCSTPLYDQGGPVEILSFLYLGSAYHASRKDMLDALGITALINVSANC
  PNHFEGHYQYKSIPVEDNHKADISSWFNEAIDFIDSIKDAGGRVFVHCQAGISRSATICLAYLMRTNRVK
  LDEAFEFVKQRRSIISPNFSFMGQLLQFESQVLAPHCSAEAGSPAMAVLDRGTSTTTVFNFPVSIPVHPT
  NSALNYLKSPITTSPSC
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013642
• ORF Size: 1101 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_013642.1, NP_038670.1
• RefSeq Size: 1933
• RefSeq ORF: 1104
• Locus ID: 19252
• Gene Summary: Dual specificity phosphatase that dephosphorylates MAP kinase MAPK1/ERK2 on both 'Thr-183' and 'Tyr-185', regulating its activity during the meiotic cell cycle. [UniProtKB/Swiss-Prot Function]