Lias (NM_024471) Mouse Tagged ORF Clone

CAT#: MG205779

Lias (GFP-tagged) - Mouse lipoic acid synthetase (Lias)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Lias
• Synonyms: 7a5ex; 2900022L22Rik; 4933425M12Rik; C77512; lip-syn; LS
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205779 representing NM_024471
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTACGCTGCTGGGATACAGCTCGCAGCCTGGGGTCCCGGATATTTGGAAGATATGCGTTCACGG
  TTAGAGCATTAAGTTCTTTGCCAGATAAGAAAAAGGAATTTTTGCATAATGGACCAGACCTTCAAGATTT
  TGTATCTGGTGATCTTGCAGACAAGAGTACATGGGATGAATATAAAGGAAACTTAAAGCGCCAGAAAGGA
  GAAAGGCTAAGACTACCTCCATGGCTAAAGACAAAGATACCCATGGGTAAAAACTACAATAAACTGAAAA
  ATACATTGCGGAATTTAAGTCTCCACACAGTGTGTGAGGAAGCCCGGTGCCCCAACATTGGAGAGTGTTG
  GGGAGGTGGGGAATATGCCACAGCCACAGCCACGATCATGTTGATGGGGGACACATGCACAAGAGGTTGC
  AGATTTTGTTCCGTTAAGACCGCAAGAAATCCCCCTCCATTGGATGCCAATGAGCCCGACAATACGGCCA
  AAGCAATTGCAGAGTGGGGTCTGGATTATGTTGTCCTGACGTCGGTGGATCGAGATGATGTGGCCGACGG
  GGGAGCTGAGCACATCGCCAAGACCGTGTCATGCTTGAAGGAAAGGAATCCAAAAATCCTCGTGGAATGC
  CTCACCCCAGACTTCCGAGGTGATCTGAGAGCAGTGGAGAAGGTGGCTCTGTCTGGATTAGATGTGTATG
  CACACAATGTGGAGACTGTCCCCGAGTTACAGAGGAAAGTTCGTGATCCCCGGGCCAATTTTGACCAGTC
  TCTTCGTGTACTGAGACATGCCAAGGAGGTCCAGCCTGATGTTGTTTCCAAAACATCGATAATGCTGGGC
  CTTGGTGAGACAGACGAGCAAGTCTATGCCACACTGAAAGCTCTCCGTGCGGCCGACGTGGACTGTTTAA
  CTCTAGGGCAGTACATGCAGCCAACGAAGCGCCACCTTAAGGTTGAAGAATATGTTACTCCTGAGAAGTT
  CAAATACTGGGAAAAAGTAGGGAATGAACTTGGATTTCTCTATACGGCAAGTGGTCCTTTGGTGCGATCT
  TCATATAAAGCAGGTGAATTTTTCCTGAAAAATCTAGTGGCTAGAAGAAAAACAAAAGCTTCTAAAGTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205779 representing NM_024471
  Red=Cloning site Green=Tags(s)
  
  MALRCWDTARSLGSRIFGRYAFTVRALSSLPDKKKEFLHNGPDLQDFVSGDLADKSTWDEYKGNLKRQKG
  ERLRLPPWLKTKIPMGKNYNKLKNTLRNLSLHTVCEEARCPNIGECWGGGEYATATATIMLMGDTCTRGC
  RFCSVKTARNPPPLDANEPDNTAKAIAEWGLDYVVLTSVDRDDVADGGAEHIAKTVSCLKERNPKILVEC
  LTPDFRGDLRAVEKVALSGLDVYAHNVETVPELQRKVRDPRANFDQSLRVLRHAKEVQPDVVSKTSIMLG
  LGETDEQVYATLKALRAADVDCLTLGQYMQPTKRHLKVEEYVTPEKFKYWEKVGNELGFLYTASGPLVRS
  SYKAGEFFLKNLVARRKTKASKV
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024471
• ORF Size: 1665 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_024471.2
• RefSeq Size: 1669
• RefSeq ORF: 1122
• Locus ID: 79464