Actg1 (BC023248) Mouse Tagged ORF Clone

CAT#: MG205832

Actg1 (GFP-tagged) - Mouse actin, gamma, cytoplasmic 1 (cDNA clone MGC:30279 IMAGE:3499390)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Actg1
• Synonyms: MGC28612, E51, Actg
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205832 representing BC023248
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGAAGAAATCGCCGCACTCGTCATTGACAATGGCTCCGGCATGTGCAAAGCCGGCTTTGCTGGCG
  ACGACGCCCCCAGGGCCGTGTTCCCTTCCATCGTAGGGCGCCCCCGACACCAGGGCGTCATGGTGGGCAT
  GGGCCAGAAAGACTCATACGTGGGTGACGAGGCCCAGAGCAAGAGGGGTATCCTGACCCTGAAGTACCCT
  ATCGAACACGGCATTGTCACTAACTGGGACGACATGGAGAAGATCTGGCACCACACCTTCTACAATGAGC
  TGCGTGTGGCTCCTGAGGAGCACCCGGTGCTTCTGACCGAGGCCCCCCTGAACCCCAAAGCTAACAGAGA
  GAAGATGACGCAGATAATGTTTGAAACCTTCAATACCCCAGCCATGTACGTGGCCATTCAGGCGGTGCTG
  TCCTTGTATGCATCTGGGCGCACCACTGGCATTGTCATGGACTCTGGTGACGGGGTCACACACACAGTGC
  CCATCTATGAGGGCTACGCCCTTCCCCACGCCATCTTGCGTCTGGACCTGGCTGGCCGGGACCTGACAGA
  CTACCTCATGAAGATCCTGACTGAACGGGGCTACAGCTTTACCACCACTGCTGAGAGGGAAATTGTTCGT
  GACATAAAGGAGAAGCTGTGCTATGTTGCCCTGGATTTTGAGCAAGAAATGGCTACTGCTGCATCATCTT
  CCTCCTTGGAGAAGAGTTACGAGCTGCCCGACGGGCAGGTGATCACCATTGGCAATGAGCGGTTCCGGTG
  TCCGGAGGCACTCTTCCAGCCTTCCTTCCTGGGCATGGAGTCCTGTGGTATCCATGAGACCACTTTCAAC
  TCCATCATGAAGTGTGATGTGGATATCCGCAAAGACCTGTATGCCAATACAGTGCTGTCTGGTGGTACCA
  CCATGTACCCAGGCATTGCTGACAGGATGCAGAAGGAGATCACAGCCCTAGCACCTAGCACGATGAAGAT
  TAAGATCATTGCTCCCCCTGAGCGCAAGTACTCAGTCTGGATCGGTGGCTCCATTCTGGCCTCACTGTCC
  ACCTTCCAGCAGATGTGGATCAGCAAGCAGGAGTATGATGAGTCAGGCCCCTCCATCGTCCACCGCAAAT
  GCTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205832 representing BC023248
  Red=Cloning site Green=Tags(s)
  
  MEEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYP
  IEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVL
  SLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVR
  DIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFN
  SIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLS
  TFQQMWISKQEYDESGPSIVHRKCF
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC023248
• ORF Size: 1125 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC023248, AAH23248
• RefSeq Size: 1931
• RefSeq ORF: 1127
• Locus ID: 11465
• Gene Summary: Actins are highly conserved proteins that are involved in various types of cell motility and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta, and gamma, have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in non-muscle cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]