B3gnt5 (NM_054052) Mouse Tagged ORF Clone

CAT#: MG205836

B3gnt5 (GFP-tagged) - Mouse UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3gnt5)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: B3gnt5
• Synonyms: beta3GnT5
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205836 representing NM_054052
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGACTGTTTGTTAGCAGAAGAGTCAAAAGATGGAAAATTTTTCACTTTTTTGTCACTTGTTTTATAT
  TAAGCTTCATGGTTTTTTGGAGCCCAATCAATAATTACATCATGAGCCATATGAAGTCCTACTCCTACAG
  ATACCTCGTAAATAGCTATGGCTTTGTAAACAATTCCCTGTCTCTCAAGCACAGCTCTGTGCAGCCTCAC
  TACCCATACTTGATCAACCACAGAGAGAAGTGTCAGGCTCAAGATGTCCTCCTCTTACTGTTTATAAAGA
  CTGCCCCTGAAAACTATGGCCGACGTTCTGCAATCAGAAAAACGTGGGGCAATGAGAACTATGTTCAGTC
  TCAACTCAATGCCAACATCAAAATTCTGTTTGCATTAGGAACTCCTGGTCCACTGAAGGGAAAAGAACTG
  CAAAAAAGACTAATCGGGGAAGATCAAGTGTACAAGGATATAATTCAGCAAGATTTCATTGATTCTTTCC
  ACAATCTTACTTCTAAATTCCTTCTTCAGTTCAGCTGGGCAAATACCTTTTGTCCACATGCCAAATTCCT
  GATGACTGCTGATGATGATATATTTATCCACATGCCAAATCTCATTGAATATCTTCAAGGGCTAGAGCAG
  ATTGGAGTTCGAGACTTTTGGATTGGTCACGTTCATCGAGGTGGCCCTCCTGTTAGGGATAAAAGCAGCA
  AATACTATGTTCCCTATGAAATGTACAAGTGGCCAGCCTACCCTGACTATACAGCTGGTGCTGCCTATGT
  TGTCTCCAGAGATGTAGCTGCCAAAATCTATGAGGCATCGCAGACACTGAATTCCAGTATGTACATAGAT
  GATGTATTCATGGGCCTCTGTGCCAATAAAGTGGGAATCTTGCCACAGGACCATGTATTTTTCTCTGGGG
  AAGGGAAAATTCCTTATCACCCCTGCATCTATGAAAAGATGATGACATCTCACGGACACTTACAAGATCT
  GCAGGACCTCTGGATAGAGGCTACACATCCTAAAGTAAAGAACATTTCAAAAGGGTTTTTTGGTCAAATA
  TACTGCAGGTTAATTAAGATAGTTCTTCTCTGCAGACTGACTTACAGGAATTCATACCCTTGTTGGGCTG
  CATTTGCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205836 representing NM_054052
  Red=Cloning site Green=Tags(s)
  
  MRLFVSRRVKRWKIFHFFVTCFILSFMVFWSPINNYIMSHMKSYSYRYLVNSYGFVNNSLSLKHSSVQPH
  YPYLINHREKCQAQDVLLLLFIKTAPENYGRRSAIRKTWGNENYVQSQLNANIKILFALGTPGPLKGKEL
  QKRLIGEDQVYKDIIQQDFIDSFHNLTSKFLLQFSWANTFCPHAKFLMTADDDIFIHMPNLIEYLQGLEQ
  IGVRDFWIGHVHRGGPPVRDKSSKYYVPYEMYKWPAYPDYTAGAAYVVSRDVAAKIYEASQTLNSSMYID
  DVFMGLCANKVGILPQDHVFFSGEGKIPYHPCIYEKMMTSHGHLQDLQDLWIEATHPKVKNISKGFFGQI
  YCRLIKIVLLCRLTYRNSYPCWAAFA
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_054052
• ORF Size: 4545 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_054052.2
• RefSeq Size: 4098
• RefSeq ORF: 1131
• Locus ID: 108105
• Gene Summary: Beta-1,3-N-acetylglucosaminyltransferase that plays a key role in the synthesis of lacto- or neolacto-series carbohydrate chains on glycolipids, notably by participating in biosynthesis of HNK-1 and Lewis X carbohydrate structures. Has strong activity toward lactosylceramide (LacCer) and neolactotetraosylceramide (nLc(4)Cer; paragloboside), resulting in the synthesis of Lc(3)Cer and neolactopentaosylceramide (nLc(5)Cer), respectively. Plays a central role in regulating neolacto-series glycolipid synthesis during embryonic development. [UniProtKB/Swiss-Prot Function]