Atp6v1c1 (NM_025494) Mouse Tagged ORF Clone

CAT#: MG205962

Atp6v1c1 (GFP-tagged) - Mouse ATPase, H+ transporting, lysosomal V1 subunit C1 (Atp6v1c1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Atp6v1c1
• Synonyms: 1700025B18Rik; U13839
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205962 representing NM_025494
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTGAGTTCTGGCTCATATCTGCTCCTGGGGAGAAAACCTGTCAGCAAACATGGGAGAAACTACATG
  CAGCGACCACCAAGAACAATAATCTTGCCGTCTCTTCCAAGTTCAACATTCCTGACCTAAAGGTTGGCAC
  GTTGGATGTCTTGGTTGGCTTGTCGGATGAACTGGCTAAACTGGATGCATTTGTAGAAGGTGTGGTCAAG
  AAAGTGGCTCAGTACATGGCTGATGTGCTGGAGGACAGCAAAGATAAAGTTCAGGAGAACTTGCTGGCCA
  GCGGAGTTGACTTGGTTACTTACATAACAAGGTTCCAGTGGGATATGGCTAAATATCCAATCAAGCAGTC
  TCTGAAAAATATTTCTGAAATAATTGCCAAGGGAGTGACTCAGATCGATAATGACCTAAAGTCTCGGGCG
  TCTGCATACAATAACCTGAAAGGGAATCTTCAGAATTTAGAGCGAAAGAACGCGGGAAGTTTGCTAACGC
  GAAGCCTGGCGGAAATCGTGAAGAAAGATGACTTTGTTCTTGATTCGGAGTATCTTGTCACATTGCTGGT
  GGTAGTTCCCAAGCTGAACCACAATGACTGGATTAAGCAGTATGAAACGCTCGCAGAAATGGTAGTACCA
  AGGTCAAGCAATGTTCTTTCAGAGGACCAAGACAGTTATCTTTGTAACGTCACCTTGTTTAGAAAAGCAG
  TTGATGATTTCAGACACAAAGCCAGAGAAAACAAGTTCATAGTTCGTGACTTCCAGTATAACGAGGAGGA
  GATGAAAGCAGATAAAGAAGAAATGACCAGGCTGTCTACTGACAAGAAGAAGCAGTTTGGACCACTTGTG
  CGGTGGCTTAAAGTGAATTTCAGTGAGGCGTTTATTGCGTGGATTCATATAAAAGCATTACGGGTTTTTG
  TGGAGTCTGTTTTAAGGTATGGCTTGCCGGTGAACTTCCAAGCAATGCTCCTTCAGCCGAATAAGAAGTC
  AGTGAAGAAGCTAAGAGAAGTGCTGCACGAGTTGTACAAGCATCTGGACAGCAGTGCGGCTGCCATCATT
  GACGCTCCCATGGATATCCCTGGCCTAAACCTGAGTCAGCAGGAATACTACCCCTATGTGTATTACAAGA
  TTGATTGCAACTTGCTGGAATTCAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205962 representing NM_025494
  Red=Cloning site Green=Tags(s)
  
  MTEFWLISAPGEKTCQQTWEKLHAATTKNNNLAVSSKFNIPDLKVGTLDVLVGLSDELAKLDAFVEGVVK
  KVAQYMADVLEDSKDKVQENLLASGVDLVTYITRFQWDMAKYPIKQSLKNISEIIAKGVTQIDNDLKSRA
  SAYNNLKGNLQNLERKNAGSLLTRSLAEIVKKDDFVLDSEYLVTLLVVVPKLNHNDWIKQYETLAEMVVP
  RSSNVLSEDQDSYLCNVTLFRKAVDDFRHKARENKFIVRDFQYNEEEMKADKEEMTRLSTDKKKQFGPLV
  RWLKVNFSEAFIAWIHIKALRVFVESVLRYGLPVNFQAMLLQPNKKSVKKLREVLHELYKHLDSSAAAII
  DAPMDIPGLNLSQQEYYPYVYYKIDCNLLEFK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025494
• ORF Size: 1146 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_025494.1, NP_079770.1
• RefSeq Size: 1488
• RefSeq ORF: 1149
• Locus ID: 66335
• Gene Summary: Subunit of the peripheral V1 complex of vacuolar ATPase. Subunit C is necessary for the assembly of the catalytic sector of the enzyme and is likely to have a specific function in its catalytic activity. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. [UniProtKB/Swiss-Prot Function]