Alg12 (NM_145477) Mouse Tagged ORF Clone

CAT#: MG206021

Alg12 (GFP-tagged) - Mouse asparagine-linked glycosylation 12 homolog (yeast, alpha-1,6-mannosyltransferase) (Alg12)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Alg12
• Synonyms: ECM39
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206021 representing NM_145477
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGGCAAGAAGTCATCGGGCAAGCGGTCATGGCCGCTTCTGGGGCTGCTGGTCACTGTAGCCACCA
  TCCACCTGGTTATATGTCCCTACACCAAGGTAGAAGAGAGCTTCAACCTTCAGGCCACACATGACCTCCT
  GTACCACCAGCTGGACATAGACAAGTACGACCACCACGAGTTTCCTGGAGTTGTTCCTAGGACATTCCTC
  GGGCCGCTGGTGATCGCAGCGTTCTCCAGCCCCGTGGTTTATGTGCTCTCGCTTTTAGAAGTATCCAAAT
  TTTATTCTCAGCTGATAGTCAGAGGAGTCCTTGGGCTTGGTGTGATTTCTGGACTCTGGACATTACAAAA
  GGAAGTGAGACAGCAGTTCGGAGCCACGGTGGCTGTCATGTTCTGCTGGATATCAGCCACACAGTTTCAT
  CTCATGTTCTACTGTACGAGGACACTCCCCAACGTGTTGGCCCTGGCTGTGGTCTTACCAGCCCTCACAG
  CCTGGCTGCAGCGGAGGTGGGCCCTGTTTGTCTGGCTCTCAGCCTTCGTCATCATTGGCTTCAGGGCTGA
  GCTGGCCATGCTGCTGGGCATTGCGCTGCTGCTGACCTTGTACCAAAGAAGACTGACGGTGGCCAGAGTG
  CTCCGACACGCCATCCCAGCAGGGCTTCTCTGTCTAGGCAAGTTGGCATCTCTGGTTTGCCTCCTGAATA
  AAAAGTCTTGGCCATACAAGGTGAGAGCTATGCTGGTGACAGGGCATATCTTGGTGAATGTGGCATATAC
  AGCCACGTCCCTCTATGTGTCTCATTTCAACTACCCTGGTGGGGTTGCGATGCAGCAGCTACATGAGCTG
  GTACCACCTCAGACAGATGTCCTTCTTCACATTGATGTGGCTGCTGCCCAGACAGGAGTGTCACGGTTTC
  TACAGGTCAATGATGACTGGAGGTATGACAAGAGTGAAGATGTGGGAGCAGCGGCCATGCTGAACTACAC
  CCACATCCTCATGGAGGCAGTGCCTGGGCACCCGGCCCTCTACAGGGACACACATCGAGTCCTGGCCAGT
  ATTGAGGGCACTACAGGTATAAGCCTGAATTTGATGAAACTGCCACCCTTTGATGTCAACCTGCAGACAA
  AGCTGGTACTTCTAGAGCGGCTGCTTAGGCCAGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206021 representing NM_145477
  Red=Cloning site Green=Tags(s)
  
  MAGKKSSGKRSWPLLGLLVTVATIHLVICPYTKVEESFNLQATHDLLYHQLDIDKYDHHEFPGVVPRTFL
  GPLVIAAFSSPVVYVLSLLEVSKFYSQLIVRGVLGLGVISGLWTLQKEVRQQFGATVAVMFCWISATQFH
  LMFYCTRTLPNVLALAVVLPALTAWLQRRWALFVWLSAFVIIGFRAELAMLLGIALLLTLYQRRLTVARV
  LRHAIPAGLLCLGKLASLVCLLNKKSWPYKVRAMLVTGHILVNVAYTATSLYVSHFNYPGGVAMQQLHEL
  VPPQTDVLLHIDVAAAQTGVSRFLQVNDDWRYDKSEDVGAAAMLNYTHILMEAVPGHPALYRDTHRVLAS
  IEGTTGISLNLMKLPPFDVNLQTKLVLLERLLRPA
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145477
• ORF Size: 1155 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_145477.1, NP_663452.1
• RefSeq Size: 1812
• RefSeq ORF: 1158
• Locus ID: 223774