Gda (BC076583) Mouse Tagged ORF Clone

CAT#: MG206054

Gda (GFP-tagged) - Mouse guanine deaminase (cDNA clone MGC:100095 IMAGE:30620457)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Gda
• Synonyms: AU015411|AW047581
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206054 representing BC076583
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGTGCGGCTCGGACGCCGCCGCTGGCGCTCGTCTTCCGAGGGACTTTCGTCCACTCCACCTGGACCT
  GCCCCATGGAGGTGCTTCGCGATCACCTCCTCGGCGTGAGCGACAGCGGCAAAATAGTGTTTCTAGAAGA
  ATCATCTCAGCAAGAAAAACTGGCCAAGGAGTGGTGCTTCAAACCATGTGAGATCAGAGAACTGAGCCAC
  CATGAGTTCTTCATGCCAGGCCTTGTTGATACCCACATCCATGCCCCTCAGTATGCCTTTGCTGGAAGCA
  ACGTTGACCTGCCGCTTTTGGAGTGGCTGAATAAGTATACATTTCCCACAGAACAAAGGTTCCGGAGCAC
  TGATGTGGCTGAAGAAGTCTACACTAGAGTTGTTAGGAGAACACTGAAGAACGGCACCACCACGGCTTGC
  TACTTTGGAACAATTCACACTGACTCATCCCTGATCCTTGCGGAAATTACAGATAAATTTGGGCAGCGAG
  CATTTGTGGGCAAAGTATGCATGGATTTGAATGATACTGTTCCAGAATACAAGGAGACCACCGAGGAGTC
  AGTCAAGGAGACAGAGAGATTTGTGTCAGAAATGCTGCAAAAGAATTATCCAAGGGTGAAACCCATAGTG
  ACCCCACGCTTTACCCTTTCTTGCACGGAGACTCTGATGAGTGAACTTGGCAACATCGCCAAGACCCATG
  ATCTGTACATCCAGAGCCATATAAGTGAAAATCGTGAAGAAATTGAAGCCGTGAAAAGCTTATACCCTAG
  TTACAAAAACTACACAGATGTCTATGATAAAAACAATCTTCTGACAAACAAGACAGTAATGGCACATGGC
  TGCTACCTTTCTGAAGAAGAACTTAACATCTTCAGTGAACGAGGAGCATCCATTGCACATTGTCCCAACT
  CTAATCTGTCGCTGAGCAGTGGCTTACTGAACGTGCTTGAGGTCCTGAAGCATAAAGTGAAGATAGGGCT
  GGGGACAGATGTGGCTGGTGGCTACTCCTATTCCATGCTTGATGCCATCAGAAGAGCAGTCATGGTTTCC
  AATGTCCTCTTAATTAATAAGGTAAATGAGAAAAACCTCACCCTCAAAGAAGTCTTCAGACTAGCCACTC
  TTGGAGGAAGCCAAGCCCTGGGGCTTGATAGCGAGATTGGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206054 representing BC076583
  Red=Cloning site Green=Tags(s)
  
  MCAARTPPLALVFRGTFVHSTWTCPMEVLRDHLLGVSDSGKIVFLEESSQQEKLAKEWCFKPCEIRELSH
  HEFFMPGLVDTHIHAPQYAFAGSNVDLPLLEWLNKYTFPTEQRFRSTDVAEEVYTRVVRRTLKNGTTTAC
  YFGTIHTDSSLILAEITDKFGQRAFVGKVCMDLNDTVPEYKETTEESVKETERFVSEMLQKNYPRVKPIV
  TPRFTLSCTETLMSELGNIAKTHDLYIQSHISENREEIEAVKSLYPSYKNYTDVYDKNNLLTNKTVMAHG
  CYLSEEELNIFSERGASIAHCPNSNLSLSSGLLNVLEVLKHKVKIGLGTDVAGGYSYSMLDAIRRAVMVS
  NVLLINKVNEKNLTLKEVFRLATLGGSQALGLDSEIG
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC076583
• ORF Size: 1163 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC076583, AAH76583
• RefSeq Size: 3432
• RefSeq ORF: 1163
• Locus ID: 14544
• Gene Summary: Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia. [UniProtKB/Swiss-Prot Function]