Ncf1 (NM_010876) Mouse Tagged ORF Clone

CAT#: MG206087

Ncf1 (GFP-tagged) - Mouse neutrophil cytosolic factor 1 (Ncf1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ncf1
• Synonyms: Ncf-1; NCF-47K; NOXO2; p47-phox; p47; p47phox
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206087 representing NM_010876
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGACACCTTCATTCGCCATATTGCCCTGCTGGGCTTCGAGAAGCGCTTCATCCCCAGCCAGCACT
  ATGTGTACATGTTCCTGGTTAAGTGGCAGGACCTGTCGGAGAAGGTGGTCTACAGAAAATTCACCGAGAT
  CTACGAGTTCCATAAAATGCTGAAGGAGATGTTCCCCATTGAGGCCGGCGAGATCCACACAGAGAACAGA
  GTCATCCCACACCTCCCGGCACCCAGGTGGTTTGATGGGCAACGAGCCGCTGAGAGTCGCCAGGGCACTC
  TCACTGAATACTTCAACGGCCTCATGGGACTGCCCGTGAAGATCTCCCGCTGCCCACACCTGCTGGACTT
  CTTCAAAGTGCGGCCTGATGACCTGAAACTGCCCACTGACAGCCAGGCGAAGAAGCCTGAGACATACCTG
  GTGCCCAAAGATGGCAAGAATAACGTAGCTGACATCACAGGCCCCATCATCCTTCAGACCTATCGGGCCA
  TTGCTGACTACGAGAAGAGTTCGGGAACAGAGATGACCGTGGCAACCGGAGACGTGGTGGACGTTGTGGA
  GAAGAGCGAGAGCGGCTGGTGGTTTTGCCAGATGAAGACAAAGCGAGGTTGGGTCCCTGCATCCTATCTG
  GAGCCCCTTGACAGTCCCGACGAGGCGGAGGATCCGGATCCCAACTACGCAGGTGAACCGTATGTAACCA
  TCAAAGCGTACGCTGCTGTTGAAGAGGACGAGATGTCCCTGTCTGAGGGTGAAGCCATTGAGGTCATTCA
  TAAGCTCCTGGATGGCTGGTGGGTGGTCAGGAAAGGGGATATCACCGGCTATTTCCCATCCATGTATCTG
  CAGAAGGCTGGGGAGGAGATAACCCAGGCCCAGCGACAGATCAGAGGCCGCGGGGCACCACCTCGAAGGT
  CGACCATCCGCAACGCACAGAGCATCCACCAGCGTTCTCGGAAGCGCCTTAGCCAGGACACCTATCGCCG
  CAACAGCGTCCGATTCCTGCAGCAGCGCAGACGCCCGGGGCGACCCGGGCCGCTGAGCACGGATGGCACA
  AAGGACAATCCATCGACTCCGCGCGTCAAACCACAGCCCGCGGTGCCTCCGCGACCCAGCTCGGACCTCA
  TCCTGCACCGCTGCACAGAGAGCACCAAACGGAAGCTGACGTCCGCTGTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206087 representing NM_010876
  Red=Cloning site Green=Tags(s)
  
  MGDTFIRHIALLGFEKRFIPSQHYVYMFLVKWQDLSEKVVYRKFTEIYEFHKMLKEMFPIEAGEIHTENR
  VIPHLPAPRWFDGQRAAESRQGTLTEYFNGLMGLPVKISRCPHLLDFFKVRPDDLKLPTDSQAKKPETYL
  VPKDGKNNVADITGPIILQTYRAIADYEKSSGTEMTVATGDVVDVVEKSESGWWFCQMKTKRGWVPASYL
  EPLDSPDEAEDPDPNYAGEPYVTIKAYAAVEEDEMSLSEGEAIEVIHKLLDGWWVVRKGDITGYFPSMYL
  QKAGEEITQAQRQIRGRGAPPRRSTIRNAQSIHQRSRKRLSQDTYRRNSVRFLQQRRRPGRPGPLSTDGT
  KDNPSTPRVKPQPAVPPRPSSDLILHRCTESTKRKLTSAV
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010876
• ORF Size: 1170 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_010876.3, NP_035006.3
• RefSeq Size: 2721
• RefSeq ORF: 1173
• Locus ID: 17969