Galk1 (NM_016905) Mouse Tagged ORF Clone

CAT#: MG206119

Galk1 (GFP-tagged) - Mouse galactokinase 1 (Galk1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Galk1
• Synonyms: AA409894; GALK; Gk; Glk; Glk1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206119 representing NM_016905
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCTTGGAGACCGCCCCGGGTCGAGGAGCTGCTGGCCGAGGCCCGCCGGGCCTTCATGGAGGAGT
  TTGGAGCCGAGCCGGAGCTGGCAGTGTCGGCGCCGGGCCGCGTCAACCTCATCGGGGAGCACACGGACTA
  CAACCAGGGCCTGGTGCTGCCCATGGCACTGGAGCTCGTGACCGTGATGGTTGGCAGCCCCCGGACAGAT
  GGGCTTGTTTCTCTTCTCACCACTTCCAAAGATGCAGACGAGCCCCAAAGACTGCAGTTCCCACTGCCCT
  CAGCCCAGTGGTCCTTGGAGCCTGGAATCCCACAGTGGGCCAATTATGTCAAGGGAGTGATTCAACATTA
  CCCAGCTTCCCCGCTCGTTGGCTTCAGTGCAGTGGTGGTCAGCTCAGTGCCCCTGGGGGGTGGGCTTTCC
  AGCTCAGCGTCTCTGGAAGTGGCCACGTACACCTTCATCCAGCAGCTCTGCCCAGACTCGGGGGCAATAG
  CTGCCCGGGCCCAGGTGTGTCAACGGGCTGAGCACAGCTTCGCAGGGGTGCCCTGTGGCATCATGGACCA
  ACTCATCGCGCTGCTGGGGCAGAAAGGCTATGCACTACTCATTGACTGCAGGTCCCTGGAAACAAGCCTG
  GTGCCACTGTCAGACCCCAAGCTGGCCGTGCTCATCACCAACTCCAATGTCCGCCATTCCCTGGGCTCCA
  GCGAGTACCCGGTTCGTCGGCGACAGTGTGAAGAAGTGGCCCAGGCCCTGGGCAAGGAGAGCCTTCGAGA
  GGTGCGGATGGAGGAGCTTGAGGCGGGCAGGGAGCTAATGAGCAAGGAGGGCTTCCGGCGGGCCCGGCAT
  GTGGTAAGCGAGATCCGGAGAACAGCCCAGGCAGCAGCTGCTATGAGCCGAGGAGACTACAAGGCCTTTG
  GGCGTCTCATGGTGGAGAGTCACTACTCACTCAGGGATGACTATGAGGTCAGCTGCCCTGAGCTGGATCA
  ATTGGTTGAGGCCGCGCTCTCTGTGCCTGGGGTTTATGGCAGTCGCATGACAGGTGGTGGCTTTGGTGGC
  TGCACCGTCACATTGCTGGAGGCCTCTGTTGCCCCTCTTGTCATAGATCATATACAGGAGCAGTACAGTG
  GGACAGCCACCTTCTACCTCTCTCAAGCTGCCGATGGAGCCCAGGTGCTGAGCTTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206119 representing NM_016905
  Red=Cloning site Green=Tags(s)
  
  MAAWRPPRVEELLAEARRAFMEEFGAEPELAVSAPGRVNLIGEHTDYNQGLVLPMALELVTVMVGSPRTD
  GLVSLLTTSKDADEPQRLQFPLPSAQWSLEPGIPQWANYVKGVIQHYPASPLVGFSAVVVSSVPLGGGLS
  SSASLEVATYTFIQQLCPDSGAIAARAQVCQRAEHSFAGVPCGIMDQLIALLGQKGYALLIDCRSLETSL
  VPLSDPKLAVLITNSNVRHSLGSSEYPVRRRQCEEVAQALGKESLREVRMEELEAGRELMSKEGFRRARH
  VVSEIRRTAQAAAAMSRGDYKAFGRLMVESHYSLRDDYEVSCPELDQLVEAALSVPGVYGSRMTGGGFGG
  CTVTLLEASVAPLVIDHIQEQYSGTATFYLSQAADGAQVLSL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016905
• ORF Size: 1176 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_016905.2, NP_058601.2
• RefSeq Size: 1506
• RefSeq ORF: 1179
• Locus ID: 14635
• Gene Summary: Major enzyme for galactose metabolism. [UniProtKB/Swiss-Prot Function]