Atg4b (NM_174874) Mouse Tagged ORF Clone

CAT#: MG206129

Atg4b (GFP-tagged) - Mouse autophagy-related 4B (yeast) (Atg4b)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Atg4b
• Synonyms: 2510009N07Rik; Apg4b; Atg4bl; Autl1; AW048066
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206129 representing NM_174874
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGCAGCCACTTTGACATATGATACTCTCCGGTTTGCTGAATTTGAAGATTTCCCTGAGACCTCAG
  AGCCTGTTTGGATTCTGGGCAGAAAATACAGCATTTTCACAGAGAAGGACGAAATCTTGTCTGATGTTGC
  ATCCAGACTTTGGTTTACATACAGGAGAAACTTTCCAGCTATTGGGGGAACTGGCCCTACTTCAGACACA
  GGCTGGGGTTGCATGCTTCGGTGTGGACAGATGATCTTTGCCCAGGCCCTGGTATGCCGGCACTTAGGTC
  GAGATTGGAGGTGGACTCAGCGGAAGAGGCAGCCTGACAGCTACTTTAATGTCCTCAATGCTTTCCTCGA
  CAGGAAGGACAGCTACTATTCCATCCATCAGATAGCGCAAATGGGAGTTGGCGAAGGCAAGTCTATAGGC
  CAGTGGTACGGGCCGAACACTGTTGCCCAGGTCCTCAAGAAACTTGCTGTATTCGACACATGGAGTTCCT
  TGGCTGTTCACATAGCAATGGACAACACTGTGGTGATGGAGGAAATCAGAAGGTTATGCAGGGCCAACCT
  TCCCTGTGCTGGGGCGGCTGCACTTCCTACTGATTCAGAGAGGCACTGTAATGGGTTCCCTGCTGGAGCT
  GAAGTCACCAACAGGCCATCGGCTTGGAGACCACTAGTGCTTCTCATCCCTCTCCGCCTGGGACTGACAG
  ACATCAATGAGGCCTATGTGGAGACGCTGAAGCACTGTTTCATGATGCCCCAGTCCCTGGGTGTTATTGG
  AGGGAAGCCCAACAGTGCCCACTACTTTATTGGCTATGTTGGGGAGGAGCTCATCTATCTGGACCCCCAC
  ACTACACAGCCTGCAGTGGAGCTGACTGACAGCTGCTTCATCCCCGACGAGAGCTTCCACTGCCAGCACC
  CTCCCTCTAGGATGGGCATTGGAGAGCTTGACCCGTCCATTGCTGTGGGGTTTTTCTGTAAGAAGGAGGA
  AGACTTTAACGACTGGTGTCAGCAAGTCAAAAAGCTATCCCAGCTTGGAGGTGCCCTGCCCATGTTTGAG
  CTGGTGGAACAGCAGCCTTCCCATCTGGCCTGCCAGGATGTCCTGAACCTGTCCCTAGACTCTTCTGATG
  TAGAGAGACTGGAGAGGTTTTTTGACTCTGAGGATGAAGACTTTGAAATCTTATCCCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206129 representing NM_174874
  Red=Cloning site Green=Tags(s)
  
  MDAATLTYDTLRFAEFEDFPETSEPVWILGRKYSIFTEKDEILSDVASRLWFTYRRNFPAIGGTGPTSDT
  GWGCMLRCGQMIFAQALVCRHLGRDWRWTQRKRQPDSYFNVLNAFLDRKDSYYSIHQIAQMGVGEGKSIG
  QWYGPNTVAQVLKKLAVFDTWSSLAVHIAMDNTVVMEEIRRLCRANLPCAGAAALPTDSERHCNGFPAGA
  EVTNRPSAWRPLVLLIPLRLGLTDINEAYVETLKHCFMMPQSLGVIGGKPNSAHYFIGYVGEELIYLDPH
  TTQPAVELTDSCFIPDESFHCQHPPSRMGIGELDPSIAVGFFCKKEEDFNDWCQQVKKLSQLGGALPMFE
  LVEQQPSHLACQDVLNLSLDSSDVERLERFFDSEDEDFEILSL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_174874
• ORF Size: 1179 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_174874.1, NP_777363.1
• RefSeq Size: 2965
• RefSeq ORF: 1182
• Locus ID: 66615