Bcat2 (NM_009737) Mouse Tagged ORF Clone

CAT#: MG206133

Bcat2 (GFP-tagged) - Mouse branched chain aminotransferase 2, mitochondrial (Bcat2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Bcat2
• Synonyms: Bcat(m); Bcat-2; Eca40
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206133 representing NM_009737
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCAGCCACACTAGGACAGGTCTGGGCCCGAAAGCTTCTGCCTGTTCCCTGGCTTCTATGTGGGT
  CCAAAAGATGTGTGTCCTCCATATTCAAGGCTGCAGACCTCCAGATTCAGATGACCAAAGAACCACAGAA
  GAAGCCAGCCCCTAGCCAGGCACTGCTGTTTGGGAAGACCTTCACAGACCACATGCTGATGGTGGAGTGG
  AATAACAAGGCTGGATGGGGCCCACCAAGGATCCAGCCCTTCCAGAACCTCACGCTACACCCAGCCTGCT
  CTGGTCTGCACTACTCTCTGCAGCTCTTTGAGGGCTTGAAAGCATACAAAGGTGGAGACCAGCAGGTACG
  CCTCTTCCGGCCCTGGCTCAACATGGACAGGATGCTACGTTCTGCTAGGCGCCTCTGCCTGCCAGACTTT
  GACAAGCAGGAGCTGTTGGAGTGTATCCGCCAGCTCATTGAAGTAGACAAAGACTGGGTTCCCGATGGCA
  ACGGAACGAGCCTCTACGTGCGGCCCGTGCTGATCGGGAATGAGCCCTCGCTGGGTGTTGGTATGGTCAC
  ACAAGCCCTCCTGTACGTCATTCTGTGCCCTGTGGGCTCCTACTTCCCTGGAGACTCCATGACACCTGTC
  TCCCTCCTGGCGGACCCTTCATTCGTCAGAGCCTGGATAGGCGGAGTCGGTGACTGCAAGTTGGGGGGGA
  ACTATGGACCCACTGTGGCTGTGCAGCGAGAAGCCCAGAAGAGAGGCTGCGAGCAGGTCCTCTGGCTGTA
  CGGGCCAGACCACCAGCTCACTGAGGTGGGCACCATGAACATCTTTGTCTACTGGACCCATGAAGACGGA
  GTACTGGAGCTGGTAACCCCCCCACTGAATGGCGTCATCTTGCCTGGAGTAGTTCGACAAAGTCTGCTGG
  ATCTGGCCAGGACTTGGGGTGAGTTCCGGGTGGCAGAACGCAAGGTCACTATGAAGGAATTGAAGCGGGC
  GCTGGAGGAAGGCCGAGTGCGAGAAGTCTTCGGCTCAGGCACTGCTTGTCAGGTCTGCCCGGTGCACCAA
  ATCCTGTATGAAGGCAAGCAACTCCACATACCTACCATGGAGAACGGCCCAGAGCTCATCCTGCGCTTCC
  AGAAGGAACTGAAGGCTATTCAGTACGGAGCCAGTGCCCACGATTGGATGTTTCGGGTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206133 representing NM_009737
  Red=Cloning site Green=Tags(s)
  
  MAAATLGQVWARKLLPVPWLLCGSKRCVSSIFKAADLQIQMTKEPQKKPAPSQALLFGKTFTDHMLMVEW
  NNKAGWGPPRIQPFQNLTLHPACSGLHYSLQLFEGLKAYKGGDQQVRLFRPWLNMDRMLRSARRLCLPDF
  DKQELLECIRQLIEVDKDWVPDGNGTSLYVRPVLIGNEPSLGVGMVTQALLYVILCPVGSYFPGDSMTPV
  SLLADPSFVRAWIGGVGDCKLGGNYGPTVAVQREAQKRGCEQVLWLYGPDHQLTEVGTMNIFVYWTHEDG
  VLELVTPPLNGVILPGVVRQSLLDLARTWGEFRVAERKVTMKELKRALEEGRVREVFGSGTACQVCPVHQ
  ILYEGKQLHIPTMENGPELILRFQKELKAIQYGASAHDWMFRV
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009737
• ORF Size: 1179 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009737.1, NP_033867.1
• RefSeq Size: 1582
• RefSeq ORF: 1182
• Locus ID: 12036
• Gene Summary: Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. May also function as a transporter of branched chain alpha-keto acids. [UniProtKB/Swiss-Prot Function]