Esam (NM_027102) Mouse Tagged ORF Clone

CAT#: MG206163

Esam (GFP-tagged) - Mouse endothelial cell-specific adhesion molecule (Esam1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Esam
• Synonyms: 2310008D05Rik; Esam1; W117m
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206163 representing NM_027102
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTCTTCAGGCTGGAACCCCCGAGACCAGCTTGCTGCGGGTTTTGTTCCTGGGACTGAGTACCCTTG
  CTGCCTTCTCCCGAGCTCAGATGGAGTTGCACGTGCCCCCGGGCCTCAACAAATTGGAAGCGGTAGAGGG
  AGAAGAAGTGGTGCTCCCCGCCTGGTACACGATGGCACGGGAGGAGTCGTGGTCCCACCCCCGGGAGGTG
  CCCATCCTGATCTGGTTCTTGGAACAAGAAGGGAAGGAACCAAACCAGGTGTTGTCTTACATTAATGGAG
  TCATGACAAATAAACCTGGAACAGCCCTGGTCCACTCTATCTCTTCACGGAATGTGTCCCTGCGCCTGGG
  GGCACTCCAGGAGGGAGACTCTGGGACTTACCGCTGTTCTGTCAATGTGCAGAATGATGAAGGCAAAAGT
  ATAGGCCACAGCATCAAAAGCATAGAGCTCAAAGTGCTGGTTCCTCCAGCTCCTCCATCCTGTAGTTTAC
  AGGGTGTACCCTATGTCGGGACCAATGTGACCCTGAACTGCAAGTCCCCAAGGAGTAAACCTACTGCTCA
  GTACCAGTGGGAGAGGCTGGCCCCATCCTCCCAGGTCTTCTTTGGACCAGCCTTAGATGCTGTTCGTGGA
  TCTTTAAAGCTCACTAACCTTTCCATTGCCATGTCTGGAGTCTATGTCTGCAAGGCTCAAAACAGAGTGG
  GCTTTGCCAAGTGCAACGTGACCTTGGACGTGATGACAGGGTCCAAGGCTGCAGTGGTCGCTGGAGCAGT
  TGTGGGCACTTTTGTTGGGTTGGTGCTGATAGCTGGGCTGGTCCTGTTGTACCAGCGCCGGAGCAAGACC
  TTGGAAGAGCTGGCCAATGATATCAAGGAAGATGCCATTGCTCCCCGGACCTTGCCTTGGACCAAAGGCT
  CAGACACAATCTCCAAGAATGGGACACTTTCTTCGGTCACCTCAGCACGAGCTCTGCGGCCACCCAAGGC
  TGCTCCTCCAAGACCTGGCACATTTACTCCCACACCCAGTGTCTCTAGCCAGGCCCTGTCCTCACCAAGA
  CTGCCCAGGGTAGATGAACCCCCACCTCAGGCAGTGTCCCTGACCCCAGGTGGGGTTTCTTCTTCTGCTC
  TGAGCCGCATGGGTGCTGTGCCTGTGATGGTGCCTGCACAGAGTCAGGCTGGGTCTCTTGTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206163 representing NM_027102
  Red=Cloning site Green=Tags(s)
  
  MILQAGTPETSLLRVLFLGLSTLAAFSRAQMELHVPPGLNKLEAVEGEEVVLPAWYTMAREESWSHPREV
  PILIWFLEQEGKEPNQVLSYINGVMTNKPGTALVHSISSRNVSLRLGALQEGDSGTYRCSVNVQNDEGKS
  IGHSIKSIELKVLVPPAPPSCSLQGVPYVGTNVTLNCKSPRSKPTAQYQWERLAPSSQVFFGPALDAVRG
  SLKLTNLSIAMSGVYVCKAQNRVGFAKCNVTLDVMTGSKAAVVAGAVVGTFVGLVLIAGLVLLYQRRSKT
  LEELANDIKEDAIAPRTLPWTKGSDTISKNGTLSSVTSARALRPPKAAPPRPGTFTPTPSVSSQALSSPR
  LPRVDEPPPQAVSLTPGGVSSSALSRMGAVPVMVPAQSQAGSLV
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_027102
• ORF Size: 1182 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_027102.3, NP_081378.1
• RefSeq Size: 1859
• RefSeq ORF: 1185
• Locus ID: 69524