Pigk (BC060175) Mouse Tagged ORF Clone

CAT#: MG206181

Pigk (GFP-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class K (cDNA clone MGC:61259 IMAGE:6827521)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pigk
• Synonyms: 3000001O05Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206181 representing BC060175
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCCCCTGCTTCCTCACTCTGCGGGTGGCTACCCTGGCCGCCTTGGCGCTCTTGTCTCTCGGCA
  GCTCTGCCGCTGGACACATCGAGGATCAAGCCGAACAGTTCTTTAGAAGTGGACACACAAATAACTGGGC
  TGTTTTGGTGTGCACATCCCGATTCTGGTTTAACTACCGACATGTTGCAAATACTCTTTCTGTTTATAGA
  AGCGTCAAGAGGCTAGGTATCCCGGACAGTCACATTGTCCTGATGCTTGCTGATGACATGGCGTGCAATG
  CTCGGAACCCCAAGCCAGCCACAGTGTTCAGCCACAAGAACATGGAGCTCAATGTGTATGGAGACGATGT
  GGAAGTGGACTACAGAAGCTATGAGGTAACTGTGGAGAACTTTTTAAGGGTATTGACCGGGAGGGTTCCA
  CCCAGTACCCCTCGCTCAAAGCGTCTTCTTTCCGACGACAGAAGCAATATCCTCATTTATATGACAGGTC
  ATGGAGGGAATGGTTTCTTGAAATTCCAAGATTCTGAAGAAATCACCAACATAGAACTTGCAGATGCGTT
  TGAACAGATGTGGCAGAAGAGACGCTACAATGAGCTGCTGTTCATTATTGACACTTGCCAGGGCGCGTCC
  ATGTACGAGCGGTTCTACTCTCCTAACATCATGGCCTTGGCTAGTAGCCAGGTGGGAGAGGATTCGCTGT
  CGCACCAGCCTGACCCTGCGATTGGAGTTCATCTTATGGATAGGTACACGTTTTATGTCTTGGAATTTTT
  GGAAGAAATTAATCCAGCTAGCCAAACTAACATGAACGACCTTTTTCAAGTGTGTCCCAAAAGTCTCTGT
  GTGTCGACCCCTGGACATCGCACTGACCTTTTCCAGCGAGATCCAAAAAATGTCCTGATCACCGATTTCT
  TCGGAAGTGTGCGCAAGGTGGAAATCACAACAGAGAAGATCAGTTTGCAGTGGGATTCACAAGTCGTGGA
  CAGCAGTTCTAAAGAAGACGGCACGGCCGAGGAGCGCATGGGACCTCTCAAGTATGCTGAGCAGCTCCCG
  GTGGCTCAGATAATACACCAGAAGCCAAAGCCGAGAGACTGGCACCCTCCCGGAGGCTTCATCCTGGGGC
  TGTGGGCGCTCATCATCATGGTCTTCTTCAAGACCTATGGGATCAAGCATATGAAGTTCATTTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206181 representing BC060175
  Red=Cloning site Green=Tags(s)
  
  MAAPCFLTLRVATLAALALLSLGSSAAGHIEDQAEQFFRSGHTNNWAVLVCTSRFWFNYRHVANTLSVYR
  SVKRLGIPDSHIVLMLADDMACNARNPKPATVFSHKNMELNVYGDDVEVDYRSYEVTVENFLRVLTGRVP
  PSTPRSKRLLSDDRSNILIYMTGHGGNGFLKFQDSEEITNIELADAFEQMWQKRRYNELLFIIDTCQGAS
  MYERFYSPNIMALASSQVGEDSLSHQPDPAIGVHLMDRYTFYVLEFLEEINPASQTNMNDLFQVCPKSLC
  VSTPGHRTDLFQRDPKNVLITDFFGSVRKVEITTEKISLQWDSQVVDSSSKEDGTAEERMGPLKYAEQLP
  VAQIIHQKPKPRDWHPPGGFILGLWALIIMVFFKTYGIKHMKFIF
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC060175
• ORF Size: 1187 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC060175, AAH60175
• RefSeq Size: 4829
• RefSeq ORF: 1187
• Locus ID: 329777