Doc2b (NM_007873) Mouse Tagged ORF Clone

CAT#: MG206484

Doc2b (GFP-tagged) - Mouse double C2, beta (Doc2b)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Doc2b
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206484 representing NM_007873
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCCTCCGGCGGCGCGGGGAGAAGGCGACCATCAGCATCCAGGAGCATATGGCCATCGACGTGTGTC
  CCGGCCCCATTCGGCCTATCAAGCAGATCTCCGATTATTTTCCCCGCTTCCCGCGGGGCCTCCCCCCTAC
  CGCCGCGCCCCGCGCCCCCGCGCCCCCGGACGCCCCCGCGCGCTCTCCCGCAGCCAGCGCCAGCCCCCGC
  AGCCCCTCCGACGGCGCCCGCGACGACGACGAAGATGTGGACCAGCTCTTCGGAGCCTACGGAGCCAGCC
  CAGGCCCCAGCCCCGGCCCCAGCCCCGCGAGGCCGCCCGCCAAGCCCCCCGAGGACGAGCCGGACGTCGA
  CGGCTACGAGTCAGACGACTGCACCGCCCTGGGTACGCTGGACTTCAGTCTGCTCTATGACCAGGAGAAC
  AACGCACTGCACTGCACCATCAGCAAGGCCAAGGGCCTGAAGCCGATGGACCACAATGGACTGGCTGATC
  CCTACGTCAAACTACACCTGCTGCCTGGAGCCAGCAAGGCAAATAAGCTCAGAACAAAAACTCTTCGGAA
  CACCCTGAACCCCTCGTGGAACGAGACCCTCACTTATTACGGAATCACGGATGAGGACATGGTCCGAAAG
  ACCCTGAGGATCTCCGTGTGTGATGAGGACAAATTCCGCCACAATGAGTTCATTGGAGAGACTCGGGTGC
  CCCTGAAGAAGCTGAAGCCCAATCACACCAAGACATTCAGCATCTGCCTGGAGAAGCAGCTGCCGGTGGA
  CAAGGCAGAGGACAAGTCTCTGGAAGAGCGAGGCCGCATCCTCATCTCCCTCAAGTACAGCTCACAGAAG
  CAGGGCCTGCTGGTGGGCATCGTGCGCTGTGCACACCTGGCTGCCATGGATGCTAATGGCTACTCGGACC
  CCTATGTGAAAACATATCTGAAGCCAGATGTAGACAAGAAATCCAAGCATAAGACAGCAGTGAAGAAGAA
  AACACTAAACCCAGAATTCAATGAGGAATTCTGTTACGAGATCAAGCATGGAGACCTGGCCAAAAAGACT
  CTGGAGGTCACTGTCTGGGATTATGACATTGGAAAATCCAATGATTTCATCGGTGGTGTGGTTCTGGGCA
  TCAACGCCAAGGGCGAGCGCCTGAAGCACTGGTTTGACTGCTTGAAGAACAAGGACAAGAGGATTGAGCG
  TTGGCACACGCTCACCAATGAGCTCCCAGGGGCTGTACTCAGCGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206484 representing NM_007873
  Red=Cloning site Green=Tags(s)
  
  MTLRRRGEKATISIQEHMAIDVCPGPIRPIKQISDYFPRFPRGLPPTAAPRAPAPPDAPARSPAASASPR
  SPSDGARDDDEDVDQLFGAYGASPGPSPGPSPARPPAKPPEDEPDVDGYESDDCTALGTLDFSLLYDQEN
  NALHCTISKAKGLKPMDHNGLADPYVKLHLLPGASKANKLRTKTLRNTLNPSWNETLTYYGITDEDMVRK
  TLRISVCDEDKFRHNEFIGETRVPLKKLKPNHTKTFSICLEKQLPVDKAEDKSLEERGRILISLKYSSQK
  QGLLVGIVRCAHLAAMDANGYSDPYVKTYLKPDVDKKSKHKTAVKKKTLNPEFNEEFCYEIKHGDLAKKT
  LEVTVWDYDIGKSNDFIGGVVLGINAKGERLKHWFDCLKNKDKRIERWHTLTNELPGAVLSD
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007873
• ORF Size: 1236 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_007873.2, NP_031899.2
• RefSeq Size: 4275
• RefSeq ORF: 1239
• Locus ID: 13447
• Gene Summary: The protein encoded by this gene is a calcium sensor involved in glucose-stimulated insulin secretion, spontaneous neurotransmitter release, and enhanced SNARE-dependent vesicle fusion. [provided by RefSeq, Sep 2015]