Bckdk (NM_009739) Mouse Tagged ORF Clone

CAT#: MG206492

Bckdk (GFP-tagged) - Mouse branched chain ketoacid dehydrogenase kinase (Bckdk)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Bckdk
• Synonyms: AI327402
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206492 representing NM_009739
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATACTGACTTCAGTGCTGGGCAGCGGCCCTCGGAGCTGGTCTTCACTTTGGCCCCTCTTGGGGTCCT
  CACTGTCACTCCGGGCTCGCTCAACATCAGCCACCGATACACACCATGTAGAGCTGGCCAGGGAACGCTC
  CAAGACTGTCACCTCTTTTTACAACCAGTCAGCCATTGATGTGGCAGCAGAGAAGCCCTCAGTTCGCCTC
  ACTCCCACCATGATGCTCTATTCCGGTCGCTCACAGGATGGCAGCCACCTTCTGAAAAGTGGCCGCTACT
  TGCAGCAAGAGTTACCAGTGAGGATTGCTCACCGCATCAAGGGCTTCCGTAGCCTTCCTTTCATCATTGG
  TTGCAACCCTACCATACTGCATGTGCACGAGCTATACATCCGGGCCTTCCAGAAGCTGACAGACTTCCCT
  CCGATCAAGGACCAGGCAGATGAGGCCCAGTACTGCCAGCTGGTGAGACAGCTGCTAGATGACCACAAGG
  ATGTGGTGACCCTGTTAGCTGAGGGTCTTCGTGAGAGCCGGAAACACATCCAGGATGAAAAGCTAGTCCG
  GTACTTCTTGGATAAAACACTGACGTCAAGACTTGGGATCCGAATGCTGGCTACTCACCACTTGGCGCTA
  CATGAAGACAAGCCTGATTTTGTTGGCATCATCTGTACTCGTCTGTCGCCCAAGAAGATTATCGAGAAGT
  GGGTGGATTTTGCCAGACGCCTGTGTGAGCACAAGTATGGCAATGCCCCTCGAGTCCGCATCAACGGACA
  TGTGGCTGCCCGGTTCCCCTTCATTCCCATGCCGCTGGACTATATCCTGCCTGAGCTGCTCAAGAATGCC
  ATGAGAGCCACAATGGAGAGTCACCTAGACACTCCCTACAATGTCCCAGATGTGGTCATCACCATCGCCA
  ATAATGATATTGATCTCATCATCAGGATCTCAGACCGGGGTGGAGGAATCGCTCATAAGGACCTGGATCG
  GGTCATGGACTACCACTTCACCACAGCTGAGGCCAGCACCCAGGACCCCCGAATCAACCCCCTCTTCGGC
  CACCTGGATATGCACAGTGGTGGCCAGTCAGGACCTATGCATGGCTTTGGCTTCGGGTTGCCCACGTCCA
  GGGCATATGCAGAGTATCTTGGTGGCTCCCTGCAGCTGCAGTCCCTGCAGGGCATTGGCACGGATGTCTA
  CCTACGGCTCCGCCACATTGATGGTCGGGAGGAAAGCTTCAGAATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206492 representing NM_009739
  Red=Cloning site Green=Tags(s)
  
  MILTSVLGSGPRSWSSLWPLLGSSLSLRARSTSATDTHHVELARERSKTVTSFYNQSAIDVAAEKPSVRL
  TPTMMLYSGRSQDGSHLLKSGRYLQQELPVRIAHRIKGFRSLPFIIGCNPTILHVHELYIRAFQKLTDFP
  PIKDQADEAQYCQLVRQLLDDHKDVVTLLAEGLRESRKHIQDEKLVRYFLDKTLTSRLGIRMLATHHLAL
  HEDKPDFVGIICTRLSPKKIIEKWVDFARRLCEHKYGNAPRVRINGHVAARFPFIPMPLDYILPELLKNA
  MRATMESHLDTPYNVPDVVITIANNDIDLIIRISDRGGGIAHKDLDRVMDYHFTTAEASTQDPRINPLFG
  HLDMHSGGQSGPMHGFGFGLPTSRAYAEYLGGSLQLQSLQGIGTDVYLRLRHIDGREESFRI
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009739
• ORF Size: 1236 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009739.3, NP_033869.1
• RefSeq Size: 2857
• RefSeq ORF: 1239
• Locus ID: 12041