Actr3b (NM_001004365) Mouse Tagged ORF Clone

CAT#: MG206628

Actr3b (GFP-tagged) - Mouse ARP3 actin-related protein 3 homolog B (yeast) (Actr3b)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Actr3b
• Synonyms: 9630005C02; Arp3b; Arp3beta; ARP11; AW047569
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206628 representing NM_001004365
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGCTCGCTGCCTCCCTGCGTGGTTGACTGCGGCACCGGGTACACCAAGCTTGGCTACGCAGGCA
  ACACTGAACCACAGTTCATTATCCCGTCATGTATCGCCATCCGAGAATCTGCAAAGGTAGTTGATCAAGC
  CCAAAGGAGGGTGTTGAGAGGAGTCGATGACCTTGATTTCTTCATAGGGGATGAAGCCATTGATAAACCT
  ACCTACGCTACCAAGTGGCCTATACGACATGGCATCGTTGAAGACTGGGATCTTATGGAAAGGTTCATGG
  AACAAGTGGTCTTTAAGTATCTTCGTGCTGAACCTGAGGACCACTACTTTTTAATGACAGAACCTCCACT
  GAACACACCAGAAAACAGAGAGTACCTTGCAGAAATCATGTTTGAATCATTTAATGTACCAGGACTCTAC
  ATTGCAGTACAGGCAGTGCTGGCTCTGGCAGCCTCTTGGACATCCCGGCAAGTTGGTGAACGCACACTAA
  CGGGGATAGTTATAGACAGTGGAGACGGTGTCACCCATGTTATCCCGGTGGCAGAAGGTTATGTAATTGG
  AAGCTGCATCAAACACATCCCAATTGCAGGTAGAGATATTACGTATTTCATTCAACAGCTGCTAAGGGAG
  AGGGAGGTGGGAATCCCTCCTGAGCAGTCACTGGAAACCGCAAAAGCCATCAAGGAGAAGTACTGCTACA
  TTTGCCCGGATATTGTCAGAGAATTTGCTAAGTATGACGTAGATCCTCGGAAGTGGATCAAACAGTACAC
  AGGGATCAATGCCATCAACCAGAAGAAGTTCATTATAGATGTTGGTTATGAAAGGTTCCTGGGACCAGAA
  ATATTCTTTCACCCTGAGTTTGCCAACCCAGACTTTATGGAGTCCATCTCGGATGTTGTCGATGAAGTCA
  TACAGAGCTGTCCCATAGATGTGCGCCGGCCGCTGTATAAGAACGTCGTTCTTTCTGGAGGCTCCACAAT
  GTTCAGAGATTTTGGACGTCGTCTGCAGAGAGATTTGAAAAGAGTGGTGGACGCCAGGTTAAAGCTGAGC
  CAAGAGCTCAGTGGAGGCAGGATAAAGCCTAAACCTGTGGAGGTGCAGGTGGTCACGCACCACATGCAGC
  GCTATGCCGTGTGGTTTGGAGGCTCGATGCTAGCCTCAACTCCGGAGTTCTTTCAGGTCTGTCACACCAA
  GAAGGACTATGAGGAATACGGCCCAAGCATCTGCCGCCACAACCCTGTCTTCGGAGTCATGTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206628 representing NM_001004365
  Red=Cloning site Green=Tags(s)
  
  MAGSLPPCVVDCGTGYTKLGYAGNTEPQFIIPSCIAIRESAKVVDQAQRRVLRGVDDLDFFIGDEAIDKP
  TYATKWPIRHGIVEDWDLMERFMEQVVFKYLRAEPEDHYFLMTEPPLNTPENREYLAEIMFESFNVPGLY
  IAVQAVLALAASWTSRQVGERTLTGIVIDSGDGVTHVIPVAEGYVIGSCIKHIPIAGRDITYFIQQLLRE
  REVGIPPEQSLETAKAIKEKYCYICPDIVREFAKYDVDPRKWIKQYTGINAINQKKFIIDVGYERFLGPE
  IFFHPEFANPDFMESISDVVDEVIQSCPIDVRRPLYKNVVLSGGSTMFRDFGRRLQRDLKRVVDARLKLS
  QELSGGRIKPKPVEVQVVTHHMQRYAVWFGGSMLASTPEFFQVCHTKKDYEEYGPSICRHNPVFGVMS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001004365
• ORF Size: 1254 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004365.1, NP_001004365.1
• RefSeq Size: 1734 bp
• RefSeq ORF: 1257 bp
• Locus ID: 242894
• Cytogenetics: 5 B1