Dnmt3l (NM_019448) Mouse Tagged ORF Clone

CAT#: MG206697

Dnmt3l (GFP-tagged) - Mouse DNA (cytosine-5-)-methyltransferase 3-like (Dnmt3l), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Dnmt3l
• Synonyms: D6Ertd14e; ecat7
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206697 representing NM_019448
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTTCCCGGGAGACACCTTCTTCTTGCTCTAAGACCCTTGAAACCTTGGACCTGGAGACTTCCGACA
  GCTCTAGCCCTGATGCTGACAGTCCTCTGGAAGAGCAATGGCTGAAATCCTCCCCAGCCCTGAAGGAGGA
  CAGTGTGGATGTGGTACTGGAAGACTGCAAAGAGCCTCTGTCCCCCTCCTCGCCTCCGACAGGCAGAGAG
  ATGATCAGGTACGAAGTCAAAGTGAACCGACGGAGCATTGAAGACATCTGCCTCTGCTGTGGAACTCTCC
  AGGTGTACACTCGGCACCCCTTGTTTGAGGGAGGGTTATGTGCCCCATGTAAGGATAAGTTCCTGGAGTC
  CCTCTTCCTGTATGATGATGATGGACACCAGAGTTACTGCACCATCTGCTGTTCCGGGGGTACCCTGTTC
  ATCTGTGAGAGCCCCGACTGTACCAGATGCTACTGTTTCGAGTGTGTGGACATCCTGGTGGGCCCCGGGA
  CCTCAGAGAGGATCAATGCCATGGCCTGCTGGGTTTGCTTCCTGTGCCTGCCCTTCTCACGGAGTGGACT
  GCTGCAGAGGCGCAAGAGGTGGCGGCACCAGCTGAAGGCCTTCCATGATCAAGAGGGAGCGGGCCCTATG
  GAGATATACAAGACAGTGTCTGCATGGAAGAGACAGCCAGTGCGGGTACTGAGCCTTTTTAGAAATATTG
  ATAAAGTACTAAAGAGTTTGGGCTTTTTGGAAAGCGGTTCTGGTTCTGGGGGAGGAACGCTGAAGTACGT
  GGAAGATGTCACAAATGTCGTGAGGAGAGACGTGGAGAAATGGGGCCCCTTTGACCTGGTGTACGGCTCG
  ACGCAGCCCCTAGGCAGCTCTTGTGATCGCTGTCCCGGCTGGTACATGTTCCAGTTCCACCGGATCCTGC
  AGTATGCGCTGCCTCGCCAGGAGAGTCAGCGGCCCTTCTTCTGGATATTCATGGACAATCTGCTGCTGAC
  TGAGGATGACCAAGAGACAACTACCCGCTTCCTTCAGACAGAGGCTGTGACCCTCCAGGATGTCCGTGGC
  AGAGACTACCAGAATGCTATGCGGGTGTGGAGCAACATTCCAGGGCTGAAGAGCAAGCATGCGCCCCTGA
  CCCCAAAGGAAGAAGAGTATCTGCAAGCCCAAGTCAGAAGCAGGAGCAAGCTGGACGCCCCGAAAGTTGA
  CCTCCTGGTGAAGAACTGCCTTCTCCCGCTGAGAGAGTACTTCAAGTATTTTTCCCAAAACTCACTTCCT
  CTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206697 representing NM_019448
  Red=Cloning site Green=Tags(s)
  
  MGSRETPSSCSKTLETLDLETSDSSSPDADSPLEEQWLKSSPALKEDSVDVVLEDCKEPLSPSSPPTGRE
  MIRYEVKVNRRSIEDICLCCGTLQVYTRHPLFEGGLCAPCKDKFLESLFLYDDDGHQSYCTICCSGGTLF
  ICESPDCTRCYCFECVDILVGPGTSERINAMACWVCFLCLPFSRSGLLQRRKRWRHQLKAFHDQEGAGPM
  EIYKTVSAWKRQPVRVLSLFRNIDKVLKSLGFLESGSGSGGGTLKYVEDVTNVVRRDVEKWGPFDLVYGS
  TQPLGSSCDRCPGWYMFQFHRILQYALPRQESQRPFFWIFMDNLLLTEDDQETTTRFLQTEAVTLQDVRG
  RDYQNAMRVWSNIPGLKSKHAPLTPKEEEYLQAQVRSRSKLDAPKVDLLVKNCLLPLREYFKYFSQNSLP
  L
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019448
• ORF Size: 1263 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_019448.2, NP_062321.1
• RefSeq Size: 1670
• RefSeq ORF: 1266
• Locus ID: 54427
• Gene Summary: CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein that is a catalytically inactive regulatory factor of DNA methyltransferases. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]