Sf3b4 (NM_153053) Mouse Tagged ORF Clone

CAT#: MG206765

Sf3b4 (GFP-tagged) - Mouse splicing factor 3b, subunit 4 (Sf3b4)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Sf3b4
• Synonyms: Sap49; SF3b49; SF3b50
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206765 representing NM_153053
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCCGGACCGATCTCCGAACGGAATCAGGATGCCACGGTGTACGTGGGCGGTCTGGACGAGAAAG
  TGAGTGAGCCGCTGCTGTGGGAGCTCTTTCTCCAGGCAGGGCCAGTGGTCAACACCCACATGCCCAAGGA
  CAGAGTCACTGGCCAGCACCAGGGCTATGGCTTTGTTGAATTCCTGAGCGAGGAAGATGCCGACTATGCC
  ATTAAGATTATGAACATGATCAAACTCTATGGAAAGCCAATACGGGTGAACAAGGCCTCAGCTCACAACA
  AAAACCTGGATGTGGGAGCCAACATTTTCATTGGAAATCTGGACCCAGAAATTGATGAAAAGCTGCTTTA
  CGATACTTTCAGCGCCTTTGGAGTCATCCTACAGACCCCCAAGATCATGCGGGACCCTGACACAGGCAAC
  TCCAAGGGTTACGCCTTCATTAATTTTGCCTCCTTCGATGCTTCGGATGCAGCAATTGAGGCCATGAACG
  GGCAGTACCTGTGTAACCGTCCTATCACTGTGTCTTATGCCTTCAAGAAGGACTCTAAGGGTGAACGCCA
  TGGCTCAGCAGCTGAAAGACTCCTGGCAGCCCAGAACCCGCTGTCCCAGGCTGACCGCCCTCACCAGCTG
  TTTGCCGATGCACCCCCTCCGCCCTCTGCCCCCAATCCCGTGGTTTCATCCCTGGGTTCTGGGCTTCCTC
  CACCAGGCATGCCGCCTCCTGGCTCTTTTCCACCTCCAGTGCCACCTCCTGGGGCCCTCCCTCCTGGGAT
  TCCCCCAGCCATGCCCCCACCACCTATGCCACCTGGGGCTGGAGGACATGGCCCCCCAGCAGCAGGGACT
  CCAGGGGCTGGACATCCCGGTCACGGACACTCACATCCTCACCCGTTCCCACCAGGTGGGATGCCCCATC
  CAGGGATGTCCCAGATGCAGCTGGCCCACCATGGCCCCCATGGCCTAGGACACCCCCATGCTGGGCCTCC
  GGGCTCTGGGGGGCAGCCACCACCTCGGCCACCTCCTGGAATGCCTCATCCTGGACCTCCTCCAATGGGC
  ATGCCCCCCCGAGGGCCTCCTTTTGGATCTCCCATGGGTCACCCAGGTCCCATGCCTCCACACGGCATGC
  GTGGGCCTCCTCCATTGATGCCCCCTCATGGATACACGGGTCCTCCAAGACCCCCTCCCTATGGCTACCA
  GCGGGGGCCCCTCCCGCCACCCAGACCCACTCCACGGCCCCCAGTTCCACCTCGTGGTCCTCTTCGGGGC
  CCACTTCCTCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206765 representing NM_153053
  Red=Cloning site Green=Tags(s)
  
  MAAGPISERNQDATVYVGGLDEKVSEPLLWELFLQAGPVVNTHMPKDRVTGQHQGYGFVEFLSEEDADYA
  IKIMNMIKLYGKPIRVNKASAHNKNLDVGANIFIGNLDPEIDEKLLYDTFSAFGVILQTPKIMRDPDTGN
  SKGYAFINFASFDASDAAIEAMNGQYLCNRPITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQL
  FADAPPPPSAPNPVVSSLGSGLPPPGMPPPGSFPPPVPPPGALPPGIPPAMPPPPMPPGAGGHGPPAAGT
  PGAGHPGHGHSHPHPFPPGGMPHPGMSQMQLAHHGPHGLGHPHAGPPGSGGQPPPRPPPGMPHPGPPPMG
  MPPRGPPFGSPMGHPGPMPPHGMRGPPPLMPPHGYTGPPRPPPYGYQRGPLPPPRPTPRPPVPPRGPLRG
  PLPQ
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153053
• ORF Size: 1272 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_153053.3, NP_694693.1
• RefSeq Size: 1697
• RefSeq ORF: 1275
• Locus ID: 107701
• Gene Summary: This gene encodes one of the subunits of splicing factor 3B. A similar gene in human encodes a protein that cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes, and also belongs with the minor U12-dependent spliceosome. [provided by RefSeq, May 2015]