Smad3 (NM_016769) Mouse Tagged ORF Clone

CAT#: MG206770

Smad3 (GFP-tagged) - Mouse MAD homolog 3 (Drosophila) (Smad3)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Smad3
• Synonyms: AU022421; Madh3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206770 representing NM_016769
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGTCCATCCTGCCCTTCACCCCCCCGATCGTGAAGCGCCTGCTGGGTTGGAAGAAGGGCGAGCAGA
  ACGGGCAGGAGGAGAAGTGGTGCGAGAAGGCGGTCAAGAGCTTGGTGAAGAAGCTCAAGAAGACGGGGCA
  GTTGGACGAGCTGGAGAAGGCCATCACCACGCAGAACGTGAACACCAAGTGCATTACCATCCCCAGGTCA
  CTGGATGGTCGGCTGCAGGTGTCCCATCGGAAGGGGCTCCCTCACGTTATCTACTGCCGCCTGTGGCGAT
  GGCCCGACCTGCACAGCCACCATGAATTACGGGCCATGGAGCTCTGTGAGTTTGCCTTCAACATGAAGAA
  GGATGAAGTGTGTGTAAATCCTTACCACTATCAGAGAGTAGAGACGCCAGTTCTACCTCCAGTGTTGGTG
  CCACGCCACACCGAGATCCCGGCCGAGTTCCCCCCACTGGATGACTACAGCCATTCCATTCCCGAGAACA
  CTAACTTCCCTGCTGGCATTGAGCCCCAGAGCAATATTCCAGAAACCCCACCTCCTGGCTACCTGAGTGA
  AGATGGAGAAACCAGTGACCACCAGATGAACCACAGCATGGACGCAGGTTCTCCAAACCTCTCCCCGAAT
  CCGATGTCCCCAGCACACAATAACTTGGACCTACAGCCAGTCACCTACTGTGAGCCGGCCTTCTGGTGCT
  CCATCTCCTACTACGAGCTGAACCAGCGAGTTGGGGAGACATTCCACGCCTCACAGCCATCCATGACAGT
  AGATGGCTTCACTGACCCCTCCAACTCGGAGCGCTTCTGCCTGGGCCTACTGTCCAATGTCAACCGGAAT
  GCAGCCGTGGAACTTACAAGGCGACACATTGGGAGAGGTGTGCGGCTCTACTACATCGGAGGGGAGGTCT
  TTGCGGAGTGCCTCAGTGACAGTGCTATTTTCGTCCAGTCTCCCAACTGCAACCAGCGCTATGGCTGGCA
  CCCGGCCACTGTCTGCAAGATCCCACCAGGCTGCAACCTGAAGATCTTCAACAACCAGGAATTTGCTGCC
  CTCCTAGCTCAGTCTGTCAACCAGGGCTTTGAGGCTGTCTACCAGCTGACGCGCATGTGCACCATCCGTA
  TGAGCTTCGTCAAAGGCTGGGGAGCAGAGTACAGGAGACAGACAGTGACCAGCACCCCCTGCTGGATTGA
  GCTACACCTGAATGGACCCTTGCAGTGGCTTGACAAGGTCCTCACCCAGATGGGTTCCCCGAGCATCCGC
  TGTTCCAGTGTGTCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206770 representing NM_016769
  Red=Cloning site Green=Tags(s)
  
  MSSILPFTPPIVKRLLGWKKGEQNGQEEKWCEKAVKSLVKKLKKTGQLDELEKAITTQNVNTKCITIPRS
  LDGRLQVSHRKGLPHVIYCRLWRWPDLHSHHELRAMELCEFAFNMKKDEVCVNPYHYQRVETPVLPPVLV
  PRHTEIPAEFPPLDDYSHSIPENTNFPAGIEPQSNIPETPPPGYLSEDGETSDHQMNHSMDAGSPNLSPN
  PMSPAHNNLDLQPVTYCEPAFWCSISYYELNQRVGETFHASQPSMTVDGFTDPSNSERFCLGLLSNVNRN
  AAVELTRRHIGRGVRLYYIGGEVFAECLSDSAIFVQSPNCNQRYGWHPATVCKIPPGCNLKIFNNQEFAA
  LLAQSVNQGFEAVYQLTRMCTIRMSFVKGWGAEYRRQTVTSTPCWIELHLNGPLQWLDKVLTQMGSPSIR
  CSSVS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016769
• ORF Size: 1275 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_016769.2, NP_058049.2
• RefSeq Size: 5091
• RefSeq ORF: 1278
• Locus ID: 17127