Syt4 (NM_009308) Mouse Tagged ORF Clone

CAT#: MG206780

Syt4 (GFP-tagged) - Mouse synaptotagmin IV (Syt4)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Syt4
• Synonyms: SytIV
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206780 representing NM_009308
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCCTATCACCACCAGCCGCGTGGAATTCGATGAAATTCCCACAGTGGTGGGCATCTTCAGTGCTT
  TTGGCCTCGTCTTCACTGTCTCTCTCTTTGCCTGGATCTGCTGTCAGAGAAGATCAGCCAAATCCAACAA
  GACTCCTCCATACAAGTTTGTGCACGTGCTTAAAGGAGTTGATATCTACCCAGAAAACCTAAGTAGCAAA
  AAGAAGTTTGGAGGAGATGACAAGAGTGAAGTGAAGGGTAAAGCCGCTCTGCCCAACCTTTCCCTGCATC
  TTGACCTAGAGAAGCGAGACCTCAATGGCAACTTCCCCAAAGCCAACCCCAAAGCTGGCAGCTCTTCTGA
  TCTGGAAAATGTCACCCCAAAGCTCTTTACGGAGACAGAAAAGGAGGCCAATTCCCCTGAGAGCTTGAAG
  TCCAGCACTTCCCTCACGTCAGAGGAGAAACAAGAGAAGCTGGGGACACTCTTCTTGTCTCTAGAGTACA
  ACTTCGAGAAGAAAGCATTTGTGGTGAACATCAAGGAAGCCCAGGGCTTACCAGCCATGGACGAGCAATC
  CATGACCTCTGACCCGTACATCAAAATGACAATCTTACCAGAGAAGAAGCACAGAGTGAAGACCAGAGTG
  CTCAGGAAGACGCTGGACCCTGTTTTTGATGAAACCTTCACATTCTATGGAATCCCTTATCCCCACATCC
  AAGAGCTCTCCTTGCACTTTACAGTTCTGAGTTTTGACAGGTTTTCTAGAGATGATGTCATTGGAGAAGT
  CCTGATTCCTCTTTCAGGGATTGAATTATCAGATGGAAAAATGTTAATGACTAGAGAGATCATCAAGAGA
  AATGCTAAGAAGTCTTCTGGCCGGGGTGAACTTCTGGTCTCTCTCTGTTATCAGTCCACTACAAACACGC
  TCACTGTGGTGGTCTTAAAAGCGCGGCACCTACCGAAATCTGATGTGTCTGGACTTTCAGATCCCTACGT
  CAAAGTGAACCTGTACCATGCCAAGAAGAGAATCTCTAAAAAGAAGACTCACGTTAAGAAATGCACTCCC
  AACGCAGTGTTCAATGAACTGTTTGTCTTTGATATTCCTTGTGAGAGTCTTGAAGAAATCAGTGTTGAAT
  TCTTGGTTTTGGATTCTGAAAGGGGATCCCGAAATGAGGTGATTGGACGGTTGGTCCTGGGTGCCACAGC
  AGAAGGAAGCGGTGGGGGGCACTGGAAGGAGATCTGTGACTTCCCCAGGAGACAAATTGCTAAGTGGCAC
  ATGCTCTGTGATGGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206780 representing NM_009308
  Red=Cloning site Green=Tags(s)
  
  MAPITTSRVEFDEIPTVVGIFSAFGLVFTVSLFAWICCQRRSAKSNKTPPYKFVHVLKGVDIYPENLSSK
  KKFGGDDKSEVKGKAALPNLSLHLDLEKRDLNGNFPKANPKAGSSSDLENVTPKLFTETEKEANSPESLK
  SSTSLTSEEKQEKLGTLFLSLEYNFEKKAFVVNIKEAQGLPAMDEQSMTSDPYIKMTILPEKKHRVKTRV
  LRKTLDPVFDETFTFYGIPYPHIQELSLHFTVLSFDRFSRDDVIGEVLIPLSGIELSDGKMLMTREIIKR
  NAKKSSGRGELLVSLCYQSTTNTLTVVVLKARHLPKSDVSGLSDPYVKVNLYHAKKRISKKKTHVKKCTP
  NAVFNELFVFDIPCESLEEISVEFLVLDSERGSRNEVIGRLVLGATAEGSGGGHWKEICDFPRRQIAKWH
  MLCDG
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009308
• ORF Size: 1275 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009308.3, NP_033334.2
• RefSeq Size: 3901
• RefSeq ORF: 1278
• Locus ID: 20983
• Gene Summary: The protein encoded by this gene belongs to the synaptotagmin family. Members of this family are multi-domained, integral membrane proteins of synaptic vesicles, and are thought to serve as Ca2+ sensors in the process of vesicular trafficking and exocytosis. This gene is primarily expressed in the nervous tissues. [provided by RefSeq, Jul 2008]