Sept11 (NM_001009818) Mouse Tagged ORF Clone

CAT#: MG206843

41528 (GFP-tagged) - Mouse septin 11 (Sept11)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Sept11
• Synonyms: 6230410I01Rik; AW548875; D5Ertd606e
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206843 representing NM_001009818
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGTGGCGGTGGGGAGACCGAGTAATGAAGAGCTGCGAAACTTATCACTTTCTGGCCATGTTGGAT
  TTGACAGTCTGCCCGACCAGCTGGTCAACAAATCCACTTCTCAAGGATTCTGTTTCAACATCCTGTGCGT
  GGGTGAGACAGGTATTGGCAAATCCACATTGATGGACACTTTATTCAACACCAAATTTGAAAGTGACCCA
  GCTACTCACAACGAGCCAGGCGTCCGGTTAAAAGCCAGAAGCTATGAACTCCAGGAAAGCAACGTACGGC
  TGAAGCTAACAATCGTTGACACAGTGGGATTTGGAGACCAGATTAATAAAGATGACAGCTATAAGCCTAT
  AGTGGAATACATTGACGCCCAGTTTGAGGCCTACTTGCAAGAAGAATTGAAAATTAAACGTTCTCTCTTC
  AACTATCATGACACAAGGATTCACGCCTGCCTTTACTTTATCGCCCCCACGGGACATTCACTGAAGTCCC
  TGGACCTGGTCACCATGAAGAAGCTGGACAGCAAGGTGAACATCATCCCGATCATCGCGAAAGCCGACAC
  CATCGCCAAAAACGAATTGCACAAATTCAAGAGTAAGATCATGAGTGAACTAGTTAGCAACGGAGTCCAG
  ATATATCAGTTCCCCACAGATGAGGAAACAGTGGCAGAGATTAACGCAACGATGAGTGTCCATCTCCCAT
  TCGCAGTGGTCGGCAGTACTGAAGAGGTCAAGATTGGCAACAAGATGGCGAAGGCCAGGCAGTACCCTTG
  GGGTGTGGTGCAGGTTGAGAACGAAAACCACTGTGATTTTGTGAAGCTGCGGGAGATGCTGATCCGAGTG
  AACATGGAGGACCTGCGAGAGCAGACGCACACTCGCCACTACGAGTTGTATCGGCGCTGCAAGCTTGAGG
  AGATGGGCTTCAAGGACACGGACCCCGACAGCAAGCCCTTCAGTCTTCAGGAGACCTACGAAGCAAAGAG
  GAACGAGTTCCTGGGGGAGCTACAGAAGAAAGAGGAGGAGATGAGACAGATGTTCGTCATGAGGGTGAAG
  GAGAAAGAGGCGGAGCTCAAGGAGGCAGAAAAAGAGCTTCATGAGAAGTTCGACCTCCTGAAGCGGACTC
  ACCAAGAGGAGAAGAAGAAGGTGGAAGATAAGAAGAAGGAGCTTGAGGAGGAGGTGAGCAACTTCCAGAA
  AAAGAAGGCGGCGGCTCAGCTCTTGCAATCCCAGGCCCAGCAGTCTGGGGCCCAGCAAACCAAGAAGGAC
  AAGGATAAGAAAAATGCAAGCTTCGCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206843 representing NM_001009818
  Red=Cloning site Green=Tags(s)
  
  MAVAVGRPSNEELRNLSLSGHVGFDSLPDQLVNKSTSQGFCFNILCVGETGIGKSTLMDTLFNTKFESDP
  ATHNEPGVRLKARSYELQESNVRLKLTIVDTVGFGDQINKDDSYKPIVEYIDAQFEAYLQEELKIKRSLF
  NYHDTRIHACLYFIAPTGHSLKSLDLVTMKKLDSKVNIIPIIAKADTIAKNELHKFKSKIMSELVSNGVQ
  IYQFPTDEETVAEINATMSVHLPFAVVGSTEEVKIGNKMAKARQYPWGVVQVENENHCDFVKLREMLIRV
  NMEDLREQTHTRHYELYRRCKLEEMGFKDTDPDSKPFSLQETYEAKRNEFLGELQKKEEEMRQMFVMRVK
  EKEAELKEAEKELHEKFDLLKRTHQEEKKKVEDKKKELEEEVSNFQKKKAAAQLLQSQAQQSGAQQTKKD
  KDKKNASFA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001009818
• ORF Size: 1287 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001009818.1, NP_001009818.1
• RefSeq Size: 5123 bp
• RefSeq ORF: 1290 bp
• Locus ID: 52398
• Cytogenetics: 5 47.29 cM