Sgk1 (NM_011361) Mouse Tagged ORF Clone

CAT#: MG206878

Sgk1 (GFP-tagged) - Mouse serum/glucocorticoid regulated kinase (Sgk)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Sgk1
• Synonyms: Sgk
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206878 representing NM_011361
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCGTCAAAGCCGAGGCTGCTCGAAGCACCCTTACCTACTCCAGAATGAGGGGAATGGTAGCGATTC
  TCATCGCTTTTATGAAACAGAGAAGGATGGGCCTGAACGATTTTATTCAGAAGATTGCCAGCAACACCTA
  TGCATGCAAACACGCTGAAGTTCAGTCCATTTTGAAAATGTCCCATCCTCAGGAGCCGGAGCTTATGAAC
  GCTAACCCCTCTCCTCCGCCAAGTCCCTCTCAACAAATCAACCTGGGTCCGTCCTCCAACCCTCACGCCA
  AACCCTCCGACTTTCACTTCTTGAAAGTGATCGGAAAGGGCAGTTTTGGAAAGGTTCTTCTGGCTAGGCA
  CAAGGCAGAAGAAGTATTCTATGCAGTCAAAGTTTTACAGAAGAAAGCCATCCTGAAGAAGAAAGAGGAG
  AAGCATATTATGTCAGAGCGGAATGTTCTGTTGAAGAATGTGAAGCACCCTTTCCTGGTGGGCCTTCACT
  TCTCATTCCAGACCGCTGACAAGCTCTACTTTGTCCTGGACTACATTAATGGTGGAGAGCTGTTCTACCA
  TCTCCAGAGGGAGCGCTGCTTCCTGGAACCACGGGCTCGCTTCTACGCAGCTGAAATAGCCAGTGCCCTG
  GGCTATCTGCACTCCCTAAACATCGTTTATAGAGACTTAAAACCTGAGAATATTCTCCTAGACTCCCAGG
  GGCACATCGTCCTCACTGACTTTGGGCTCTGCAAAGAGAATATTGAGCATAACGGGACAACATCTACCTT
  CTGTGGCACGCCTGAGTATCTGGCTCCTGAGGTCCTCCATAAACAGCCGTATGACCGGACGGTGGACTGG
  TGGTGTCTTGGGGCTGTCCTGTATGAGATGCTCTACGGCCTGCCCCCGTTTTATAGCCGGAACACGGCTG
  AGATGTACGACAATATTCTGAACAAGCCTCTCCAGTTGAAACCAAATATTACAAACTCGGCAAGGCACCT
  CCTGGAAGGCCTCCTGCAGAAGGACCGGACCAAGAGGCTGGGTGCCAAGGATGACTTTATGGAGATTAAG
  AGTCATATTTTCTTCTCTTTAATTAACTGGGATGATCTCATCAATAAGAAGATTACACCCCCATTTAACC
  CAAATGTGAGTGGGCCCAGTGACCTTCGGCACTTCGATCCCGAGTTTACCGAGGAGCCGGTCCCCAGCTC
  CATCGGCAGGTCCCCTGACAGCATCCTTGTCACGGCCAGTGTGAAGGAAGCAGCAGAAGCCTTCCTCGGC
  TTCTCCTATGCACCTCCTGTGGATTCCTTCCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206878 representing NM_011361
  Red=Cloning site Green=Tags(s)
  
  MTVKAEAARSTLTYSRMRGMVAILIAFMKQRRMGLNDFIQKIASNTYACKHAEVQSILKMSHPQEPELMN
  ANPSPPPSPSQQINLGPSSNPHAKPSDFHFLKVIGKGSFGKVLLARHKAEEVFYAVKVLQKKAILKKKEE
  KHIMSERNVLLKNVKHPFLVGLHFSFQTADKLYFVLDYINGGELFYHLQRERCFLEPRARFYAAEIASAL
  GYLHSLNIVYRDLKPENILLDSQGHIVLTDFGLCKENIEHNGTTSTFCGTPEYLAPEVLHKQPYDRTVDW
  WCLGAVLYEMLYGLPPFYSRNTAEMYDNILNKPLQLKPNITNSARHLLEGLLQKDRTKRLGAKDDFMEIK
  SHIFFSLINWDDLINKKITPPFNPNVSGPSDLRHFDPEFTEEPVPSSIGRSPDSILVTASVKEAAEAFLG
  FSYAPPVDSFL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011361
• ORF Size: 1293 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_011361.1, NP_035491.1
• RefSeq Size: 2429
• RefSeq ORF: 1296
• Locus ID: 20393
• Gene Summary: This gene encodes a serine/threonine protein kinase that plays an important role in cellular stress response. This kinase activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. This enzyme is activated by protein phosphorylation and degraded via the ubiquitination and proteasome pathway. Multiple transcript variants encoding different isoforms have been found for this gene. A pseudogene of this gene was identified on chromosome 12. [provided by RefSeq, Sep 2009]