Alg1 (BC011281) Mouse Tagged ORF Clone

CAT#: MG206977

Alg1 (GFP-tagged) - Mouse asparagine-linked glycosylation 1 homolog (yeast, beta-1,4-mannosyltransferase) (cDNA clone MGC:18946

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Alg1
• Synonyms: HMT1, HMAT1, MGC18946
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206977 representing BC011281
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCGTCCTGCGTGGCGCTGCTGGTCCTGGCGCTGCTGTTGCTGGTGCTGCTGCTGGGCTTGTGGA
  AGCGTGGGCGGCAAACGGGCAGGGCCCGGCATATGGTCGTCGTGGTGCTGGGCGACGTGGGCCGCAGCCC
  CCGCATGCAGTACCACGCGCTGTCATTGGCCCAGAGCGGCTTTTCGGTGACGCTGCTGGGGTTCTACACT
  GGGCCCCGAATTCTCCAGTATGGAGTCAAGGTTGTATTTCAGGCAGTGTACTTGCTGTGGAAGATGATGC
  GCATGGACCCTGCAGCCTACATCTTCCTCCAGAACCCTCCAGGTCTGCCTGCTATTGCCGTCTGCTGGTT
  TGTGGGCTGCATTTGTGGGAGCAAATTGGTCATCGACTGGCACAACTATGGCTACTCCATCATGGGCTTG
  GTCCATGGCCCCCGCCACCCCATTGTGCTGCTGGCCAAATGGTATGAGAAGTTCTTCGGGCGCCTGTCCC
  ACCTGAACCTGTGTGTGACCAATGCTATGCGGGAGGACCTGGCAGAGAACTGGTGTGTCAGGGCTGTGAC
  GCTCTACGACAAGCCAGCATCTTTCTTTAAGGAGACACCCCTGGACCTGCAGCATGAACTCTTTATGAAG
  CTGAGCCACACGTACTCTCCTTTCCAGAGCTGCTCAGATCCCTCACATCCTGACACAGAGAGGTCGGCCT
  TCACTGAGAGGGATTGTCAGAGCGGGGTTGTGAGGCGTCTGCATGGGCGGCCAGCACTGCTCGTGAGCAG
  CACAAGCTGGACAGAGGACGAGGACTTCTCCATCCTGCTTCGAGCATTAGAAAAGTTTGAGCAACAAGCA
  CTCACTGGAGACAGCCTCCCTTCCCTCGTCTGTGTGATAACAGGGAAGGGACCGCTCAGAGAGCACTACC
  GCCACCTCATCAGCCAGAAGCACCTTAGGCACATCCGGTTCTGCACCCCATGGCTGGAAGCCGAGGACTA
  CCCACTGCTTCTAGGGTCAGCGGATCTGGGTGTCTGTCTGCACATGTCCTCCAGTGGTCTGGACCTGCCC
  ATGAAGGTGGTGGACATGTTTGGGTGCCACTTGCCTGTGTGTGCCGTGAACTTCAAGTGTCTGCACGAGC
  TTGTAAGACATGGAGAGAATGGTCTGGTCTTCAAGGATGCTGAGGAGCTGGCAGCTCAGCTGCAGATGCT
  TTTCTCAAAGTTCCCAGACCCTGCTGGAAAGCTAAGCCAGTTCCGGAAGAAACTGCAGGAGTCGAGGCAG
  CAACGCTGGGATGAGAGCTGGCAGCACACTGTGCTCCCTCTGCTCACTCACAGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206977 representing BC011281
  Red=Cloning site Green=Tags(s)
  
  MAASCVALLVLALLLLVLLLGLWKRGRQTGRARHMVVVVLGDVGRSPRMQYHALSLAQSGFSVTLLGFYT
  GPRILQYGVKVVFQAVYLLWKMMRMDPAAYIFLQNPPGLPAIAVCWFVGCICGSKLVIDWHNYGYSIMGL
  VHGPRHPIVLLAKWYEKFFGRLSHLNLCVTNAMREDLAENWCVRAVTLYDKPASFFKETPLDLQHELFMK
  LSHTYSPFQSCSDPSHPDTERSAFTERDCQSGVVRRLHGRPALLVSSTSWTEDEDFSILLRALEKFEQQA
  LTGDSLPSLVCVITGKGPLREHYRHLISQKHLRHIRFCTPWLEAEDYPLLLGSADLGVCLHMSSSGLDLP
  MKVVDMFGCHLPVCAVNFKCLHELVRHGENGLVFKDAEELAAQLQMLFSKFPDPAGKLSQFRKKLQESRQ
  QRWDESWQHTVLPLLTHR
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC011281
• ORF Size: 1316 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC011281
• RefSeq Size: 1677
• RefSeq ORF: 1316
• Locus ID: 208211