Gpr177 (BC018381) Mouse Tagged ORF Clone

CAT#: MG207034

Gpr177 (GFP-tagged) - Mouse G protein-coupled receptor 177 (cDNA clone MGC:25946 IMAGE:4237619)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Gpr177
• Synonyms: 5031439A09Rik|AI173978|AI987742|Gpr177
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207034 representing BC018381
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGATGAGTCCATGGTTCCAGTTTATGCTGTTTATCTTGCAGCTAGACATTGCTTTCAAGCTGAACA
  ACCAAATCAGAGAAAATGCAGAAATTTCCATGGATGTTTCCCTGGGTTACCGTGATGATATGTTTTCTGA
  ATGGACTGAAATGGCCCATGAAAGAGTACCACGTAAACTCAAATGTACTTTTACATCCCCCAAGACCCCA
  GAGCACGAAGGTCGTTATTATAATTGTGATGTCCTTCCTTTCATGGAAATTGGGTCAGTGGCTCATAAGT
  ATTACCTTCTAAATATCCGGCTACCTGTAAATGAGAAAAAGAAAATCAATGTTGGAATTGGGGAGATAAA
  GGACATTCGGTTGGTGGGAATCCACCAAAACGGAGGTTTCACTAAGGTATGGTTTGCTATGAAGACCTTC
  CTTACACCCAGCATCTTCATCATTATGGTGTGGTATTGGAGGAGGATCACCATGATGTCCCGACCTCCAG
  TGCTTCTGGAAAAAGTCATCTTTGCCCTTGGGATTTCCATGACCTTTATCAATATCCCTGTGGAATGGTT
  TTCTATTGGATTTGACTGGACCTGGATGCTGTTATTTGGTGACATCCGACAGGGCATCTTCTATGCAATG
  CTTCTTTCTTTCTGGATCATCTTCTGTGGCGAGCACATGATGGATCAACATGAACGGAATCACATTGCAG
  GGTACTGGAAGCAAGTTGGACCAATTGCTGTTGGCTCCTTCTGCCTCTTCATATTTGACATGTGTGAAAG
  AGGAGTACAACTCACAAATCCTTTCTACAGTATCTGGACTACAGATGTTGGAACAGAACTGGCTATGGCT
  TTCATCATTGTGGCAGGTATCTGCCTCTGCCTCTATTTCCTGTTTCTGTGCTTCATGGTATTTCAGGTAT
  TTAGAAACATCAGTGGGAAGCAGTCTAGCCTCCCAGCCATGAGCAAAGTCCGGAGGCTGCACTATGAGGG
  TCTGATCTTCAGGTTCAAGTTCCTCATGCTTATCACCTTGGCTTGTGCTGCCATGACTGTTATCTTCTTC
  ATCGTTAGTCAGGTGTCAGAAGGCCATTGGAAATGGGGTGGTGTCACAGTTCAAGTGAGCAGTGCTTTCT
  TCACTGGAATCTATGGGATGTGGAACCTGTATGTCTTTGCTTTGATGTTCTTGTATGCACCATCCCATAA
  GAACTATGGGGAAGACCAGTCTAATGGTGACCTGGGTGTCCACAGCGGGGAAGAACTGCAGCTTACTACC
  ACTATAACCCATGTAGATGGACCAACTGAGATCTACAAGTTGACCCGTAAAGAAGCACAGGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207034 representing BC018381
  Red=Cloning site Green=Tags(s)
  
  MEMSPWFQFMLFILQLDIAFKLNNQIRENAEISMDVSLGYRDDMFSEWTEMAHERVPRKLKCTFTSPKTP
  EHEGRYYNCDVLPFMEIGSVAHKYYLLNIRLPVNEKKKINVGIGEIKDIRLVGIHQNGGFTKVWFAMKTF
  LTPSIFIIMVWYWRRITMMSRPPVLLEKVIFALGISMTFINIPVEWFSIGFDWTWMLLFGDIRQGIFYAM
  LLSFWIIFCGEHMMDQHERNHIAGYWKQVGPIAVGSFCLFIFDMCERGVQLTNPFYSIWTTDVGTELAMA
  FIIVAGICLCLYFLFLCFMVFQVFRNISGKQSSLPAMSKVRRLHYEGLIFRFKFLMLITLACAAMTVIFF
  IVSQVSEGHWKWGGVTVQVSSAFFTGIYGMWNLYVFALMFLYAPSHKNYGEDQSNGDLGVHSGEELQLTT
  TITHVDGPTEIYKLTRKEAQE
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC018381
• ORF Size: 1325 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC018381, AAH18381
• RefSeq Size: 2188
• RefSeq ORF: 1325
• Locus ID: 68151