Bckdha (NM_007533) Mouse Tagged ORF Clone

CAT#: MG207048

Bckdha (GFP-tagged) - Mouse branched chain ketoacid dehydrogenase E1, alpha polypeptide (Bckdha)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Bckdha
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207048 representing NM_007533
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGCGGCCAAGATCTGGAGGCCGAGCCGTGGCCTGCGCCAGGCTGCTCTTCTCCTGTTGGGACGAT
  CTGGGGTTCGGGGCTTGGCTAGATCTCACCCCAGCAGGCAGCAGCAACAGCAGTTCCCATCCCTGGACGA
  CAAGCCCCAGTTCCCAGGGGCCTCTGCAGAGTTTGTAGACAAGCTTGAGTTCATCCAGCCCAATGTCATC
  TCCGGCATCCCCATCTACCGTGTCATGGACCGCCAGGGCCAGATCATCAACCCCAGTGAAGATCCCCACC
  TGCCCCAGGAGGAGGTGCTGAAGTTCTACCGGAGCATGACGCTGCTCAACACCATGGACCGCATTCTCTA
  TGAGTCCCAGCGGCAGGGCCGGATCTCCTTCTACATGACCAACTATGGCGAGGAGGGGACACATGTGGGC
  AGTGCCGCTGCCTTGGAGCGCACAGACCTGGTGTTTGGCCAGTACCGGGAGGCAGGTGTGCTCATGTACC
  GGGACTACCCGCTGGAGCTGTTCATGTCCCAGTGCTACGGCAATGTGAATGACCCAGGCAAGGGACGCCA
  GATGCCTGTTCACTACGGCTGCAAGGAACGCCACTTCGTCACCATTTCCTCTCCACTGGCCACGCAGATC
  CCTCAGGCGGTAGGGGCAGCCTATGCTGCCAAGCGGGCCAATGCCAACCGGATTGTGATCTGTTACTTTG
  GCGAGGGGGCAGCCAGTGAAGGGGATGCCCACGCCGGTTTCAATTTCGCTGCCACCCTGGAGTGTCCCAT
  CATCTTCTTCTGCCGGAACAATGGCTATGCCATCTCCACACCAACCTCTGAGCAGTACCGTGGGGATGGC
  ATAGCGGCTCGGGGCCCTGGGTACGGCATCATGTCAATCCGTGTGGACGGCAACGATGTGTTTGCTGTGT
  ACAATGCCACTAAGGAGGCCCGACGGCGGGCTGTGGCTGAGAACCAGCCCTTCCTCATTGAGGCCATGAC
  CTACAGGATTGGCCACCACAGCACCAGTGACGACAGCTCAGCGTACCGTTCGGTGGACGAGGTCAATTAC
  TGGGACAAGCAGGACCACCCAATCTCGAGGCTGAGGCAGTACCTGCTGAACCAGGGTTGGTGGGATGAGG
  AACAGGAGAAGGCCTGGCGGAAGCAGTCACGAAAGAAGGTCATGGAAGCCTTTGAGCAGGCTGAGCGGAA
  GCTAAAGCCGAACCCAAGCCTCCTCTTCTCCGATGTGTACCAGGAGATGCCCGCCCAGCTCCGCAGGCAG
  CAGGAGTCGCTGGCACGGCACCTGCAGACTTACGGGGAGCACTACCCCCTGGACCACTTTGACAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207048 representing NM_007533
  Red=Cloning site Green=Tags(s)
  
  MSAAKIWRPSRGLRQAALLLLGRSGVRGLARSHPSRQQQQQFPSLDDKPQFPGASAEFVDKLEFIQPNVI
  SGIPIYRVMDRQGQIINPSEDPHLPQEEVLKFYRSMTLLNTMDRILYESQRQGRISFYMTNYGEEGTHVG
  SAAALERTDLVFGQYREAGVLMYRDYPLELFMSQCYGNVNDPGKGRQMPVHYGCKERHFVTISSPLATQI
  PQAVGAAYAAKRANANRIVICYFGEGAASEGDAHAGFNFAATLECPIIFFCRNNGYAISTPTSEQYRGDG
  IAARGPGYGIMSIRVDGNDVFAVYNATKEARRRAVAENQPFLIEAMTYRIGHHSTSDDSSAYRSVDEVNY
  WDKQDHPISRLRQYLLNQGWWDEEQEKAWRKQSRKKVMEAFEQAERKLKPNPSLLFSDVYQEMPAQLRRQ
  QESLARHLQTYGEHYPLDHFDK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007533
• ORF Size: 1326 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_007533.2, NP_031559.2
• RefSeq Size: 1739
• RefSeq ORF: 1341
• Locus ID: 12039