Spred1 (BC079606) Mouse Tagged ORF Clone

CAT#: MG207088

Spred1 (GFP-tagged) - Mouse sprouty protein with EVH-1 domain 1, related sequence (cDNA clone MGC:90633 IMAGE:30355455)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Spred1
• Synonyms: 5730461F13Rik|AL024345|AW047647
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207088 representing BC079606
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGAGGAGACGGCGACTTCTGACAACGATAATAGTTATGCACGAGTGCGAGCTGTGGTGATGACCA
  GAGATGACTCAAGTGGTGGATGGCTACCACTCGGAGGGAGCGGACTGAGCAGCGTCACTGTCTTCAGAGT
  CCCTCATCAGGAAGAGAATGGCTGTGCGGACTTTTTTATCCGTGGAGAGAGACTCAGAGACAAAATGGTG
  GTTTTGGAATGTATGCTTAAGAAAGACCTCATCTATAATAAGGTCACTCCCACATTTCACCACTGGAAGA
  TCGATGACAAGAAGTTTGGCCTTACCTTTCAGAGTCCTGCTGATGCCAGGGCTTTTGATCGAGGCATTCG
  AAGAGCTATAGAGGATATATCTCTAGGGTGCCCAGCGTCAAAAACTGAGGCTGAAGGAGGAGATGATGAT
  TTACAAACGACTGAAGAAGACACTTCCCGTTCCCTAGTGAAAGATCACTTTTTCCAGCAAGAGACAGTTG
  TTACCAGTGAACCTTACAGAAGCTCAGACATAAGACCGTTACCCTTTGAAGATCTGAATGCCAGAAGAGT
  CTACTTGCAAAGCCAAGTCAGCCAGATACCATTCAGTCAGCAAGGCCTAGACATTCAGAGCCGAAGTATG
  GAATATGTACAGCGGCAAATATCTAAGGAATGTGGGAGCCTAAAGTCCCAAACTAGGGTCCCTTTGAAGT
  CAATCAGACATGTCAGCTTTCAAGATGAGGATGAGATTGTTAGAATAAATCCTCGAGATATCTTAATACG
  TCGATATGCAGACTACAGACATCCGGACATGTGGAAAAATGACTTGGAGAGAGATGATACTGACTCCAGT
  GTTCCATTTTCTAAACAAGACAGTAAAAAGTCAGACTATCTCTACCACTGTGGGGATGAGACTAAGTTAA
  GTTCTCTGAAAGACTCTGTGGTATTTAAGACACAGCCTCCCTCGTTAAAATTTAAGTCAAAACGAAGAAA
  AGAGGATGGGGAACGTTCTCGCTGCGTATACTGCCAGGAAAGGTTTAATCATGAAGAAAACGCCAGGGGG
  AAATGCCAGGACGCTCCAGATCCTGTTAAAAGATGCATATATCAAGTTAGCTGCATGCTCTGTGCTGAGA
  GCATGCTATATCATTGTATGTCAGACTCAGAGGGAGACTTTTCTGATCCTTGTTCGTGTGACACTAGCGA
  TGACAAGTTCTGCCTAAGGTGGTTAGCCCTGGTAGCTCTGTCTTTCATTGTACCATGTATGTGCTGCTAC
  GTCCCTTTGAGAATGTGCCATCGCTGTGGTGAGGCATGCGGGTGCTGTGGTGGGAAACATAAGGCTGCTG
  GG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207088 representing BC079606
  Red=Cloning site Green=Tags(s)
  
  MSEETATSDNDNSYARVRAVVMTRDDSSGGWLPLGGSGLSSVTVFRVPHQEENGCADFFIRGERLRDKMV
  VLECMLKKDLIYNKVTPTFHHWKIDDKKFGLTFQSPADARAFDRGIRRAIEDISLGCPASKTEAEGGDDD
  LQTTEEDTSRSLVKDHFFQQETVVTSEPYRSSDIRPLPFEDLNARRVYLQSQVSQIPFSQQGLDIQSRSM
  EYVQRQISKECGSLKSQTRVPLKSIRHVSFQDEDEIVRINPRDILIRRYADYRHPDMWKNDLERDDTDSS
  VPFSKQDSKKSDYLYHCGDETKLSSLKDSVVFKTQPPSLKFKSKRRKEDGERSRCVYCQERFNHEENARG
  KCQDAPDPVKRCIYQVSCMLCAESMLYHCMSDSEGDFSDPCSCDTSDDKFCLRWLALVALSFIVPCMCCY
  VPLRMCHRCGEACGCCGGKHKAAG
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC079606
• ORF Size: 1334 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC079606, AAH79606
• RefSeq Size: 3042
• RefSeq ORF: 1334
• Locus ID: 114715
• Gene Summary: This gene encodes a membrane-associated protein that is phosphorylated by tyrosine kinases in response to growth factors. The encoded protein acts as a negative regulator of the mitogen-activated protein (MAP) kinase signaling pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]