Clu (NM_013492) Mouse Tagged ORF Clone

CAT#: MG207148

Clu (GFP-tagged) - Mouse clusterin (Clu)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Clu
• Synonyms: AI893575; ApoJ; Cli; D14Ucla3; Sgp-2; Sgp2; SP-40; Sugp-2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207148 representing NM_013492
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGATTCTCCTGCTGTGCGTGGCACTGCTGCTGATCTGGGACAATGGCATGGTCCTGGGAGAGCAGG
  AGGTCTCTGACAATGAGCTCCAAGAACTGTCCACTCAAGGGAGTAGGTATATTAATAAGGAGATTCAGAA
  CGCCGTCCAGGGAGTGAAGCACATAAAAACTCTCATAGAAAAAACCAACGCAGAGCGCAAGTCCTTGCTC
  AACAGTTTAGAGGAAGCCAAGAAGAAGAAAGAGGATGCTCTGGAGGACACTAGGGATTCTGAAATGAAGC
  TGAAGGCATTCCCGGAAGTGTGTAACGAGACCATGATGGCCCTCTGGGAGGAGTGCAAGCCCTGCCTGAA
  GCATACCTGCATGAAGTTCTATGCACGTGTCTGCAGGAGCGGCTCAGGGCTGGTCGGCCAGCAGCTAGAG
  GAGTTTCTAAACCAGAGCTCACCCTTCTACTTCTGGATGAACGGCGACCGCATCGACTCCCTGCTGGAGA
  GTGACCGGCAGCAGAGCCAAGTCCTGGATGCCATGCAGGACAGCTTTGCTCGGGCATCTGGCATCATAGA
  CACGCTCTTTCAGGACCGGTTCTTCGCCCGTGAGCTTCATGACCCCCACTACTTCTCCCCCATCGGCTTC
  CCACACAAGAGGCCTCATTTCTTATATCCCAAGTCCCGCTTGGTCCGCAGCCTCATGTCTCCCTCCCACT
  ATGGGCCTCCGAGCTTCCACAACATGTTCCAGCCTTTCTTTGAGATGATCCACCAGGCTCAACAGGCCAT
  GGATGTCCAGCTCCACAGCCCAGCCTTCCAGTTCCCAGACGTGGATTTCTTAAGAGAAGGTGAAGATGAC
  CGCACTGTGTGCAAGGAGATCCGCCGCAACTCCACAGGATGCCTGAAGATGAAGGGCCAGTGTGAAAAGT
  GCCAGGAGATCTTGTCTGTGGACTGTTCAACCAACAATCCTGCCCAGGCTAACCTGCGCCAGGAGCTGAA
  CGACTCGCTCCAGGTGGCCGAGAGGCTGACAGAGCAGTACAAGGAGCTGCTGCAGTCCTTCCAGTCGAAG
  ATGCTCAACACCTCATCCCTGCTGGAGCAGCTGAACGACCAGTTCAACTGGGTGTCCCAGCTGGCTAACC
  TCACACAGGGCGAAGACAAGTACTACCTTCGGGTCTCCACCGTGACCACCCATTCCTCTGACTCAGAGGT
  CCCCTCCCGTGTCACTGAGGTGGTGGTGAAGCTGTTTGACTCTGACCCCATCACAGTGGTGTTACCAGAA
  GAAGTCTCTAAGGATAACCCTAAGTTTATGGACACAGTGGCGGAGAAGGCGCTACAGGAATACCGCAGGA
  AAAGCCGTGCGGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207148 representing NM_013492
  Red=Cloning site Green=Tags(s)
  
  MKILLLCVALLLIWDNGMVLGEQEVSDNELQELSTQGSRYINKEIQNAVQGVKHIKTLIEKTNAERKSLL
  NSLEEAKKKKEDALEDTRDSEMKLKAFPEVCNETMMALWEECKPCLKHTCMKFYARVCRSGSGLVGQQLE
  EFLNQSSPFYFWMNGDRIDSLLESDRQQSQVLDAMQDSFARASGIIDTLFQDRFFARELHDPHYFSPIGF
  PHKRPHFLYPKSRLVRSLMSPSHYGPPSFHNMFQPFFEMIHQAQQAMDVQLHSPAFQFPDVDFLREGEDD
  RTVCKEIRRNSTGCLKMKGQCEKCQEILSVDCSTNNPAQANLRQELNDSLQVAERLTEQYKELLQSFQSK
  MLNTSSLLEQLNDQFNWVSQLANLTQGEDKYYLRVSTVTTHSSDSEVPSRVTEVVVKLFDSDPITVVLPE
  EVSKDNPKFMDTVAEKALQEYRRKSRAE
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013492
• ORF Size: 1344 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_013492.1, NP_038520.1
• RefSeq Size: 1669
• RefSeq ORF: 1347
• Locus ID: 12759
• Gene Summary: The protein encoded by this gene is a secreted chaperone that can, under some stress conditions, also be found in the cell cytosol. It has been suggested to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders. The encoded preproprotein undergoes proteolytic processing to generate a disulfide-linked heterodimeric mature protein comprised of alpha and beta subunits. Mice lacking the encoded protein exhibit increased severity of autoimmune myocarditis, faster progression of the acute inflammation to myocardial scarring and decreased brain injury following neonatal hypoxic-ischemic injury. [provided by RefSeq, Nov 2015]