Idh2 (NM_173011) Mouse Tagged ORF Clone

CAT#: MG207208

Idh2 (GFP-tagged) - Mouse isocitrate dehydrogenase 2 (NADP+), mitochondrial (Idh2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Idh2
• Synonyms: E430004F23; Idh-2; IDPm
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207208 representing NM_173011
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGCTACCTGCGGGCTGTGAGCTCGCTCTGCAGAGCCTCGGGCTCAGCGCGGACCTGGGCACCGG
  CAGCACTGACTGTCCCCAGCTGGCCGGAGCAGCCGCGGCGCCACTATGCTGAGAAGAGGATCAAGGTGGA
  GAAGCCGGTAGTGGAGATGGACGGTGACGAGATGACCCGGATCATCTGGCAGTTCATCAAGGAGAAGCTC
  ATCCTGCCTCACGTGGATGTTCAGCTCAAGTATTTTGACCTTGGGCTTCCAAACCGTGACCAGACCAATG
  ACCAGGTCACCATTGACTCTGCTCTGGCCACCCAGAAGTACAGTGTGGCTGTCAAGTGTGCCACAATCAC
  CCCTGATGAGGCCCGTGTGGAAGAGTTCAAGCTGAAGAAAATGTGGAAGAGCCCTAACGGAACGATCCGG
  AACATCCTTGGGGGAACCGTCTTCAGAGAGCCAATCATCTGCAAAAACATCCCCCGCCTTGTCCCTGGCT
  GGACCAAGCCCATCACCATTGGCAGGCACGCCCATGGCGACCAGTACAAGGCCACAGATTTTGTGGTAGA
  TCGAGCTGGCACGTTCAAGTTGGTCTTCACCCCAAAGGATGGCAGCAGTGCCAAGGAGTGGGAGGTGTAT
  AACTTCCCTGCCGGAGGCGTTGGGATGGGGATGTACAACACCGACGAGTCCATTTCGGGCTTCGCGCACA
  GCTGCTTCCAGTACTCTATCCAGAAGAAATGGCCGCTCTACTTGAGCACCAAGAACACCATTCTGAAGGC
  CTATGACGGGCGTTTCAAGGACATCTTCCAGGAGATCTTTGACAAGCACTATAAGACTGACTTCGACAAG
  AATAAGATCTGGTATGAACATCGGCTCATCGACGACATGGTGGCCCAGGTGCTCAAGTCTTCCGGTGGCT
  TTGTGTGGGCTTGCAAAAACTATGATGGAGACGTGCAGTCTGACATCCTGGCTCAAGGCTTTGGCTCCCT
  CGGCCTGATGACATCTGTGCTGGTCTGCCCTGATGGGAAGACAATTGAGGCTGAGGCTGCTCATGGGACA
  GTCACCCGCCATTACCGAGAACACCAGAAGGGCCGGCCCACCAGTACCAACCCTATTGCCAGCATCTTTG
  CCTGGACACGGGGTCTGGAGCATCGTGGGAAGCTGGATGGGAACCAGGACCTTATCAGGTTTGCACAGAC
  GCTGGAGAAGGTGTGCGTGCAGACTGTGGAGAGCGGAGCGATGACCAAGGACCTGGCTGGCTGTATCCAT
  GGCCTCAGCAATGTGAAGCTGAACGAGCACTTCCTGAACACCACAGACTTCCTGGACACCATTAAGAGCA
  ACCTGGACAGAGCTCTGGGCAAGCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207208 representing NM_173011
  Red=Cloning site Green=Tags(s)
  
  MAGYLRAVSSLCRASGSARTWAPAALTVPSWPEQPRRHYAEKRIKVEKPVVEMDGDEMTRIIWQFIKEKL
  ILPHVDVQLKYFDLGLPNRDQTNDQVTIDSALATQKYSVAVKCATITPDEARVEEFKLKKMWKSPNGTIR
  NILGGTVFREPIICKNIPRLVPGWTKPITIGRHAHGDQYKATDFVVDRAGTFKLVFTPKDGSSAKEWEVY
  NFPAGGVGMGMYNTDESISGFAHSCFQYSIQKKWPLYLSTKNTILKAYDGRFKDIFQEIFDKHYKTDFDK
  NKIWYEHRLIDDMVAQVLKSSGGFVWACKNYDGDVQSDILAQGFGSLGLMTSVLVCPDGKTIEAEAAHGT
  VTRHYREHQKGRPTSTNPIASIFAWTRGLEHRGKLDGNQDLIRFAQTLEKVCVQTVESGAMTKDLAGCIH
  GLSNVKLNEHFLNTTDFLDTIKSNLDRALGKQ
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_173011
• ORF Size: 1356 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_173011.2, NP_766599.2
• RefSeq Size: 1718
• RefSeq ORF: 1359
• Locus ID: 269951
• Gene Summary: Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex. [UniProtKB/Swiss-Prot Function]