Casp9 (NM_015733) Mouse Tagged ORF Clone

CAT#: MG207247

Casp9 (GFP-tagged) - Mouse caspase 9 (Casp9)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Casp9
• Synonyms: AI115399; APAF-3; AW493809; CASP-9; Caspase-9; ICE-LAP6; Mch6
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207247 representing NM_015733
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGAGGCGGACCGGCAGCTCCTGCGGCGATGCAGGGTGCGCCTAGTGAGCGAGCTGCAAGTCGCGG
  AGCTCTGGGACGCTCTGCTGAGTCGAGAGCTCTTCACGCGCGACATGATCGAGGATATTCAGCAGGCAGG
  ATCTGGGTCTCGGCGGGATCAGGCCAGGCAGCTGGTCACAGACCTTGAGACCCGAGGGAGGCAGGCCCTT
  CCTCTCTTCATCTCCTGCTTAGAGGACACAGGCCAAGGCACCCTGGCTTCACTCTTGCAAAGCGGTCGGC
  AAGCAGCCAAGCAGGATCCAGAGGCTGTTAAACCCCTAGACCACCTGGTGCCTGTGGTCCTGGGACCAAT
  GGGACTCACAGCAAAGGAGCAGAGAGTAGTGAAGCTGGACCCGTCACAGCCTGCCGTGGGAAACCTCACC
  CCAGTGGTGCTGGGGCCAGAAGAGCTCTGGCCTGCTCGGCTCAAGCCAGAGGTTCTCAGACCAGAAACAC
  CCAGGCCCGTGGACATTGGTTCTGGCGGAGCTCATGATGTCTGTGTTCCAGGGAAGATCAGGGGACATGC
  AGATATGGCATACACCCTGGATTCGGATCCCTGTGGCCACTGCCTCATCATCAACAATGTGAACTTCTGC
  CCTTCCTCGGGGCTCGGCACACGCACGGGCTCCAACTTGGACCGTGACAAACTCGAGCACCGATTCCGCT
  GGCTGCGCTTCATGGTGGAGGTGAAGAACGACCTGACTGCCAAGAAAATGGTCACGGCTTTGATGGAGAT
  GGCACACCGGAACCACCGTGCCCTGGACTGCTTTGTGGTGGTCATCCTCTCTCATGGCTGCCAGGCCAGC
  CACCTCCAGTTCCCGGGTGCTGTCTATGGGACAGATGGATGCTCCGTGTCCATTGAGAAAATTGTGAATA
  TCTTCAACGGGAGCGGCTGCCCCAGCCTGGGAGGGAAGCCCAAGCTCTTCTTCATCCAGGCCTGCGGTGG
  TGAGCAGAAAGACCATGGCTTTGAGGTGGCCTGCACTTCCTCTCAAGGCAGGACCTTGGACAGTGACTCT
  GAGCCAGATGCTGTCCCCTATCAGGAAGGCCCAAGGCCCTTGGACCAGCTGGATGCTGTGTCAAGTTTGC
  CTACCCCCAGTGACATCCTTGTGTCCTACTCCACCTTCCCAGGTTTTGTCTCCTGGAGGGACAAGAAAAG
  TGGCTCCTGGTACATCGAGACCTTGGATGGCATTCTGGAGCAGTGGGCTCGCTCTGAAGACCTGCAGTCC
  CTCCTTCTCAGGGTTGCCAATGCTGTTTCTGCGAAAGGGACTTACAAGCAGATTCCTGGCTGTTTTAACT
  TCCTCCGGAAAAAGCTGTTTTTTAAAACTTCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207247 representing NM_015733
  Red=Cloning site Green=Tags(s)
  
  MDEADRQLLRRCRVRLVSELQVAELWDALLSRELFTRDMIEDIQQAGSGSRRDQARQLVTDLETRGRQAL
  PLFISCLEDTGQGTLASLLQSGRQAAKQDPEAVKPLDHLVPVVLGPMGLTAKEQRVVKLDPSQPAVGNLT
  PVVLGPEELWPARLKPEVLRPETPRPVDIGSGGAHDVCVPGKIRGHADMAYTLDSDPCGHCLIINNVNFC
  PSSGLGTRTGSNLDRDKLEHRFRWLRFMVEVKNDLTAKKMVTALMEMAHRNHRALDCFVVVILSHGCQAS
  HLQFPGAVYGTDGCSVSIEKIVNIFNGSGCPSLGGKPKLFFIQACGGEQKDHGFEVACTSSQGRTLDSDS
  EPDAVPYQEGPRPLDQLDAVSSLPTPSDILVSYSTFPGFVSWRDKKSGSWYIETLDGILEQWARSEDLQS
  LLLRVANAVSAKGTYKQIPGCFNFLRKKLFFKTS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015733
• ORF Size: 1362 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_015733.4, NP_056548.2
• RefSeq Size: 3896
• RefSeq ORF: 1365
• Locus ID: 12371
• Gene Summary: This gene is part of a family of caspases, aspartate-specific cysteine proteases well studied for their involvement in immune and apoptosis signaling. This protein, the initiator caspase, is activated after cytochrome c release from mitochondria and targets downstream effectors. In mouse, deficiency of this gene can cause perinatal lethality. This protein may have a role in normal brain development. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Apr 2013]