Osbp2 (BC031794) Mouse Tagged ORF Clone

CAT#: MG207271

Osbp2 (GFP-tagged) - Mouse oxysterol binding protein 2 (cDNA clone MGC:25110 IMAGE:4501119)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Osbp2
• Synonyms: ORP-4, OSBPL1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207271 representing BC031794
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGACTCTACATCTTTCATCACCGTAGTCACTGAGGCCAAGGAAGACAGAAAGCCTGAGAGTGGAC
  CTGGGACCACCACTGTGGACTGGACCTCAGCAGATAATGTATTAGATGGTGCCTCATTCATGCCCAAGAA
  TTCATGCAAAGTTAAGAGGCGAGTCCGCATCCCTGACAAACCCAACTATAGCCTTAACCTCTGGAGCATC
  ATGAAGAATTGTATTGGCCGAGAGCTTTCCCGGATCCCCATGCCGGTGAACTTCAATGAGCCCCTGTCCA
  TGCTCCAGCGACTTACAGAGGACCTGGAGTACCACCACCTGCTGGACAAGGCGGTGAACTGCACCAGCTC
  GGTGGAGCAGATGTGCCTGGTAGCCGCCTTTTCTGTGTCCTCCTACTCCACCACGGTGCACCGCATCGCC
  AAGCCCTTCAACCCTATGCTCGGGGAGACCTTCGAGCTGGACCGTATGGAGGACATGGGCCTGCGTTCCC
  TCTGTGAGCAGGTGAGCCACCACCCCCCGTCTGCTGCCCACCACGTGTTCTCTAAGCATGGCTGGAGCCT
  CTGGCAAGAAATCACCATCGCCAGCAAGTTCCGAGGGAAATACATCTCTATCATGCCACTCGGTGCCATC
  CACCTAGAATTCCAGGCCAGTGGCAATCACTACGTGTGGAGGAAGAGCACCTCCACCGTGCACAACATCA
  TCGTGGGCAAGCTCTGGATTGACCAGTCAGGGGACATTGAGATTGTGAACCACAAGACCAAGGACCGGTG
  CCAGCTGAAGTTCGTACCCTACAGCTACTTCTCCAAAGAGGCAGCCCGAAAGGTGACTGGAGTGGTGAGT
  GACAGCCAGGGCAAGGCCCACTACGTGCTGTCAGGTTCGTGGGATGACCAGATGGAATGTTCTAAGATTG
  TGCACAGCAGCCCCAGCTCTGATGGGAGACAGAAAACCGTGTACCAGACACTGCCCGCCAAACTGCTCTG
  GAGGAAATACCCACTGCCGGAGAATGCGGAGAACATGTACTATTTCTCCGAGCTAGCCCTGACCCTCAAC
  GAGCAGGAGGACGGCGTGGCGCCCACCGACAGTCGCCTGCGGCCAGACCAGCGGCTTATGGAGAGGGGAC
  GCTGGGACGAGGCCAACACCGAGAAGCAACGGCTGGAGGAGAAGCAGCGCCTGTCCAGGCGACGGCGGCT
  GGAGTCATGCACGGCAGGCTGCGGTGGGGAGGAAGAGAAGGAGTCAGATGGCTATGTGCCGCTCTGGTTC
  GAGAAGAGGCTGGACCCGCTGACTGGGGAGATGGCCTGCATGTACAAGGGCGGCTACTGGGAGGCCAAGG
  AGAAGAAGGACTGGCACATGTGCCCCAACATCTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207271 representing BC031794
  Red=Cloning site Green=Tags(s)
  
  MEDSTSFITVVTEAKEDRKPESGPGTTTVDWTSADNVLDGASFMPKNSCKVKRRVRIPDKPNYSLNLWSI
  MKNCIGRELSRIPMPVNFNEPLSMLQRLTEDLEYHHLLDKAVNCTSSVEQMCLVAAFSVSSYSTTVHRIA
  KPFNPMLGETFELDRMEDMGLRSLCEQVSHHPPSAAHHVFSKHGWSLWQEITIASKFRGKYISIMPLGAI
  HLEFQASGNHYVWRKSTSTVHNIIVGKLWIDQSGDIEIVNHKTKDRCQLKFVPYSYFSKEAARKVTGVVS
  DSQGKAHYVLSGSWDDQMECSKIVHSSPSSDGRQKTVYQTLPAKLLWRKYPLPENAENMYYFSELALTLN
  EQEDGVAPTDSRLRPDQRLMERGRWDEANTEKQRLEEKQRLSRRRRLESCTAGCGGEEEKESDGYVPLWF
  EKRLDPLTGEMACMYKGGYWEAKEKKDWHMCPNIF
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC031794
• ORF Size: 1365 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC031794.1
• RefSeq Size: 3123
• RefSeq ORF: 1367
• Locus ID: 74309
• Gene Summary: The protein encoded by this gene belongs to the oxysterol-binding protein-related family of proteins, which are defined by a C-terminal sterol domain with a highly conserved EQVSHHPP motif. Oxysterols are oxygenated derivatives of cholesterol that are involved in mechanisms that include apoptosis, cholesterol homeostasis, lipid trafficking and cell differentiation. This protein is selectively expressed at high levels in the brain and testis. Within the testis, the mRNA is localized to postmeiotic germ cells, including spermatids and spermatozoa, but is not detectable in somatic cells. Mice homozygous mutant for a targeted deletion in this gene do not exhibit overt developmental phenotypes but are male sterile. Females display normal fertility. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]